Technical Resources

12430 - DMEM, high glucose, HEPES

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	30.0	0.4
L-Arginine hydrochloride	211.0	84.0	0.39810428
L-Cystine 2HCl	313.0	63.0	0.20127796
L-Glutamine	146.0	584.0	4.0
L-Histidine hydrochloride-H2O	210.0	42.0	0.2
L-Isoleucine	131.0	105.0	0.8015267
L-Leucine	131.0	105.0	0.8015267
L-Lysine hydrochloride	183.0	146.0	0.7978142
L-Methionine	149.0	30.0	0.20134228
L-Phenylalanine	165.0	66.0	0.4
L-Serine	105.0	42.0	0.4
L-Threonine	119.0	95.0	0.79831934
L-Tryptophan	204.0	16.0	0.078431375
L-Tyrosine disodium salt dihydrate	261.0	104.0	0.39846742
L-Valine	117.0	94.0	0.8034188
Vitamins
Choline chloride	140.0	4.0	0.028571429
D-Calcium pantothenate	477.0	4.0	0.008385744
Folic Acid	441.0	4.0	0.009070295
Niacinamide	122.0	4.0	0.032786883
Pyridoxine hydrochloride	206.0	4.0	0.019417476
Riboflavin	376.0	0.4	0.0010638298
Thiamine hydrochloride	337.0	4.0	0.011869436
i-Inositol	180.0	7.2	0.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	200.0	1.8018018
Ferric Nitrate (Fe(NO3)3"9H2O)	404.0	0.1	2.4752476E-4
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	97.67	0.8139166
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	3700.0	44.04762
Sodium Chloride (NaCl)	58.0	4750.0	81.89655
Sodium Phosphate monobasic (NaH2PO4-H2O)	138.0	125.0	0.9057971
Other Components
D-Glucose (Dextrose)	180.0	4500.0	25.0
HEPES	238.0	5958.0	25.033613
Phenol Red	376.4	15.0	0.039851222

Reference:

1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396.

11995 - DMEM, high glucose, pyruvate
Cat No: 11995040 , 11995065 , 11995073 , 11995081 , 11995115 , 11995123