Technical Resources

Terrific Broth

INTENDED USE: A buffered, enriched medium to obtain high yields of plasmid DNA from transformed E. coli (1). INSTRUCTIONS FOR USE: Suspend 47 g in 1 L of demineralized water. Add 4 ml of glycerol. Autoclave for 15 min at 121°C. RECOMMENDED STORAGE CONDITION: 15°C TO 30°C.

ComponentsMolecular WeightConcentration (mg/L)mM
Inorganic Salts
Dipotassium hydrogen phosphate174.1769.40.0539684
Potassium dihydrogen phosphate136.0862.20.016166247
Other Components
SELECT Peptone 140 (pancreatic digest of casein)11.8Infinity
SELECT Yeast Extract autolyzed low sodium23.6Infinity

Reference:

1. Tartof, K.D. and Hobbs, C.A. (1987) Focus® 9:2, 12.

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