Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75.0 | 30.0 | 0.4 |
| L-Alanine | 89.0 | 25.0 | 0.28089887 |
| L-Alanyl-L-Glutamine | 203.0 | 812.0 | 4.0 |
| L-Arginine hydrochloride | 211.0 | 84.0 | 0.39810428 |
| L-Asparagine (freebase) | 150.0 | 28.4 | 0.18933333 |
| L-Aspartic acid | 133.0 | 30.0 | 0.22556391 |
| L-Cystine | 313.0 | 70.0 | 0.22364217 |
| L-Glutamic Acid | 147.0 | 75.0 | 0.5102041 |
| L-Histidine hydrochloride-H2O | 210.0 | 42.0 | 0.2 |
| L-Isoleucine | 131.0 | 105.0 | 0.8015267 |
| L-Leucine | 131.0 | 105.0 | 0.8015267 |
| L-Lysine hydrochloride | 183.0 | 146.0 | 0.7978142 |
| L-Methionine | 149.0 | 30.0 | 0.20134228 |
| L-Phenylalanine | 165.0 | 66.0 | 0.4 |
| L-Proline | 115.0 | 40.0 | 0.3478261 |
| L-Serine | 105.0 | 42.0 | 0.4 |
| L-Threonine | 119.0 | 95.0 | 0.79831934 |
| L-Tryptophan | 204.0 | 16.0 | 0.078431375 |
| L-Tyrosine disodium salt | 225.0 | 104.0 | 0.46222222 |
| L-Valine | 117.0 | 94.0 | 0.8034188 |
| Vitamins | |||
| Biotin | 244.0 | 0.013 | 5.327869E-5 |
| Choline chloride | 140.0 | 4.0 | 0.028571429 |
| D-Calcium pantothenate | 477.0 | 4.0 | 0.008385744 |
| Folic Acid | 441.0 | 4.0 | 0.009070295 |
| Niacinamide | 122.0 | 4.0 | 0.032786883 |
| Pyridoxal hydrochloride | 204.0 | 4.0 | 0.019607844 |
| Riboflavin | 376.0 | 0.4 | 0.0010638298 |
| Thiamine hydrochloride | 337.0 | 4.0 | 0.011869436 |
| Vitamin B12 | 1355.0 | 0.013 | 9.594096E-6 |
| i-Inositol | 180.0 | 7.2 | 0.04 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2-2H2O) | 111.0 | 219.0 | 1.972973 |
| Magnesium Sulfate (MgSO4-7H2O) | 120.0 | 200.0 | 1.6666666 |
| Potassium Chloride (KCl) | 75.0 | 330.0 | 4.4 |
| Potassium Nitrate (KNO3) | 101.0 | 0.076 | 7.524752E-4 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 3024.0 | 36.0 |
| Sodium Chloride (NaCl) | 58.0 | 4500.0 | 77.586205 |
| Sodium Phosphate monobasic (NaH2PO4-2H2O) | 138.0 | 141.0 | 1.0217391 |
| Sodium Selenite (Na2SeO3-5H20) | 263.0 | 0.017 | 6.4638785E-5 |
| Other Components | |||
| D-Glucose (Dextrose) | 180.0 | 4500.0 | 25.0 |
| HEPES | 238.0 | 5958.0 | 25.033613 |
| Phenol Red | 376.4 | 15.0 | 0.039851222 |
| Sodium Pyruvate | 110.0 | 110.0 | 1.0 |
1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396.
2. Iscove, N.N. and Melchers, F. (1978) J. Exper. Med., 147:923.
3. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) Virology 12:185.