GSK-3 Antibody (MA3-038) in WB

Western blot analysis of GSK-3 was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. GSK-3 (alpha and beta isoforms) was detected at ~51kD and ~47kD using a GSK-3 monoclonal antibody (Product ## MA3-038) at a dilution of 1:1000 in 5% Milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product ## 31430) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product ## 34078) and the myECL Imager (Product ## 62236).

Western blot analysis of GSK-3 was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. GSK-3 (alpha and beta isoforms) was detected at ~51kD and ~47kD using a GSK-3 monoclonal antibody (Product ## MA3-038) at a dilution of 1:1000 in 5% Milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product ## 31430) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product ## 34078) and the myECL Imager (Product ## 62236).

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