Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator.

Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator (M36008). Live 3T3 cells were treated with FeTCPP, a superoxide scavenger, (bottom) or left untreated (top). Cells were then labeled with MitoSOX™ Red reagent, which fluoresces when oxidized by superoxide, and nuclei were stained with blue-fluorescent Hoechst 33342. The mitochondria of untreated cells exhibited red fluorescence, indicating the presence of superoxide, whereas the mitochondria of treated cells showed minimal fluorescence.

Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator (M36008). Live 3T3 cells were treated with FeTCPP, a superoxide scavenger, (bottom) or left untreated (top). Cells were then labeled with MitoSOX™ Red reagent, which fluoresces when oxidized by superoxide, and nuclei were stained with blue-fluorescent Hoechst 33342. The mitochondria of untreated cells exhibited red fluorescence, indicating the presence of superoxide, whereas the mitochondria of treated cells showed minimal fluorescence.

Related Products

Catalog # Name Size Price (USD) Qty
M36008 MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging, 10 vials x 50 μg Each
414.65

Online exclusive

442.00
Save 27.35 (6%)

Related Images

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›

Oxidative stress analysis in BPAE and Raw cells using CellROX® Deep Red Reagent (Cat. No. C10422) Go ›

BPAE detection of oxidative stress using MitoTracker® Green (Cat. No. M7514) and CellROX® Deep Red Reagent (Cat. No. C10422) Go ›

REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›

BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›

Agarose gel containing camptothecin-treated HL-60 cells. Go ›

Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›

Live cell imaging with CellLight™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›