Cryo-EM University: Image Formation and Aberrations

Image formation and CTF

4:04

Lenses and Ray-diagrams

Overview of lenses through Ray diagrams.
7:56

How a Lens Forms an Image

General principles of how a lens forms an image.
51:39

Phase Contrast

Qualitative explanation of phase contrast.
37:57

Amplitude Contrast

Overview of amplitude contrast.
2:51

Diffraction Contrast

Theoretical overview of diffraction contrast.
26:33

Energy Filtered Contrast

Overview energy filters and concepts on how they work.

Aberrations

6:47

Spherical Aberrations

Discussion and review of spherical lens aberrations.
0:00

Chromatic Aberrations

Discussion and review of chromatic aberrations.
0:00

Coma

Review of aberrations that play a major role in transmission electron microscopes and coma.
0:00

Rotation Center and Coma

Theoretical overview of the rotation center alignment and coma.
0:00

Optimizing Rotation Center and Coma

Demonstration of optimizing rotation center and coma in the TEM User Interface.

For Research Use Only. Not for use in diagnostic procedures.