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For critical research, you need reliable protein detection, and we provide a complete suite of labeling tools for any level of target abundance. Our fluorescent products portfolio is the most peer-referenced in life science research.
Check out this simple guide and see which tool is right for your protein.
- High-abundance target—primary conjugate, no amplification needed
- Medium- to high-abundance target—secondary conjugate, modest amplification
- Medium- to low-abundance target—streptavidin conjugate, elevate the signal
- Low-abundance target—enzyme amplification, complex process for maximum signal enhancement
Immunofluorescence selection guide
 High-abundance target e.g., tubulin |  Medium-abundance target e.g., golgi or mitochondria |  Low-abundance target e.g., receptors or cell junction | |
|---|---|---|---|
Labeled primary |  |  |  |
Secondary |  |  |  |
Streptavidin |  |  |  |
TSA |  |  |  |
 | Optimum signal for brightness/sensitivity |  | Signal too strong |  | Signal too weak |
Primary antibodies
 | Going direct with your choice of label has never been easier. We offer a wide variety of primary antibodies against key signaling proteins, cellular markers, and organelles, as well as cell junctions and CD markers. Primary antibodies provide direct detection for high-abundance targets, and high target specificity when using amplified detection methods. |
Secondary antibodies
 | Secondary antibodies with a choice of dyes provide that extra signal and assay flexibility. We offer access to some of the most cited secondary antibodies in life science research, including Alexa Fluor™ dye–labeled probes and Qdot® conjugates, as well as classic dyes in a wide range of colors. |
Streptavidin
 | Streptavidin conjugates with a choice of labels boost your signal from low-abundance targets. For improved detection sensitivity, streptavidin-based amplification techniques are widely used in fluorescence imaging to detect primary and secondary antibodies, ligands, and toxins, or DNA probes for in situ hybridization. |
TSA technology
 | Simple amplification protocols with a choice of wavelengths for rare targets. With Invitrogen™ SuperBoost™ tyramide signal amplification technology, you can see and measure low-abundance targets that are not detectable by conventional means. Tyramide-based signal amplification provides superior sensitivity without compromising resolution. |
Resources
Molecular Probes™ Handbook
| BioProbes Journal article |