Plasmid DNA Isolation

Purify plasmid DNA for every application

Our wide range of plasmid DNA extraction products are designed to isolate plasmid DNA at the purity and scale you need. Choose from our selection of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities. Learn how to manually purify plasmid DNA using our kits in our plasmid isolation protocol videos.

Learn more about plasmid DNA purity grades

Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of any protein expression workflow. Learn how to obtain transfection-grade plasmids and use them with Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Transfection-grade and advanced transfection-grade plasmid purification kits include “low-endotoxin” and “endotoxin-free” kits. These kits are used to extract plasmid DNA (pDNA) with very low levels of endotoxins from bacterial cultures. High amounts of endotoxins are released from lysed bacteria during the plasmid purification process. These are lipopolysaccharides that limit cell growth, reduce cell viability, decrease transfection efficiency and protein expression, and mimic sepsis. Removal of these endotoxins is critical to maintaining the integrity of isolated plasmid DNA.

Our low-endotoxin and endotoxin-free Invitrogen and GeneJET plasmid DNA isolation kits are ideal for researchers who need fast and simple plasmid purification that provides high purity and yield for sensitive downstream applications tailored to a specific prep size. 

Learn more about Low-Endotoxin Plasmid DNA Purification and Isolation Kits
Learn more about Endotoxin-Free Plasmid DNA Purification and Isolation Kits

For fast, simple, and reliable plasmid purification, our Invitrogen and GeneJET molecular-grade plasmid DNA (pDNA) isolation kits produce pure, ready-to-use plasmids that are compatible with a range of standard molecular biology applications. Molecular-grade kits are suitable for transformation of bacterial and yeast cells; however, they may not be ideal for transfection of sensitive mammalian cells due to the presence of endotoxins. For transfection applications, please see the transfection-grade plasmid purification kits above.