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AccuPrime DNA Polymerases
Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime technology is available in Taq, Pfx, and GC-Rich formulations.
Overview of AccuPrime technology
The thermostable AccuPrime accessory proteins enhance specific primer–template hybridization during every cycle of PCR, preventing mispriming and improving PCR specificity and yield. In addition, AccuPrime enzymes are designed with antibodies that are specific to the DNA polymerase, inhibiting its activity at room temperature and providing an automatic hot start.
How AccuPrime technology works.
Comparison of Platinum and AccuPrime DNA polymerases
Properties of Platinum DNA polymerases and AccuPrime DNA polymerases are compared for their use in high-fidelity PCR, hot-start PCR, and GC-rich PCR.
| Platinum SuperFi II DNA Polymerase | AccuPrime Taq DNA Polymerase, High Fidelity | AccuPrime Pfx DNA Polymerase | |
|---|---|---|---|
| Fidelity (vs. Taq) | >300x | 9x | 26x |
| Hot-start modification | |||
| Universal primer annealing | No | No | |
| Amplification range | 20 kb* | ≤20 kb (with Buffer II) | ≤12 kb |
| Amplicon overhang | Blunt | 3′A and blunt | Blunt |
| GC-rich format | No | No | |
| Inhibitor tolerance | No | No | |
| DNA synthesis rate | 15–30 sec/kb | 60 sec/kb | 60 sec/kb |
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | Not tested | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | Two buffers (based on template type) | One buffer |
| Product formats | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless | Stand alone: Colorless |
* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading
| Platinum II Taq DNA Polymerase | AccuPrime Taq DNA Polymerase System | |
|---|---|---|
| Hot-start modification | ||
| Fidelity (vs. Taq) | 1x | 2x |
| Amplification range | ≤5 kb | ≤5 kb |
| Inhibitor tolerance | No | |
| DNA synthesis rate | 15 sec/kb | 60 sec/kb |
| Amplicon overhang | 3′A | 3′A |
| Universal primer annealing | No | |
| Residual bacterial gDNA | ≤1 copy/enzyme unit | Not tested |
| GC-rich format | No | |
| Multiplexing | Up to 15 | Up to 20 |
| Buffer system | One optimized buffer (GC enhancer supplied in a separate vial) | Two buffers (based on template type) |
| Product formats | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless |
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading
| Platinum SuperFi II DNA Polymerase | Platinum II Taq DNA Polymerase | AccuPrime GC-Rich DNA Polymerase | |
|---|---|---|---|
| GC-rich amplification | >65% GC | >65% GC | >65% GC |
| Hot-start modification | No | ||
| Fidelity (vs. Taq) | >300x | 1x | 2x |
| Amplification range | 20 kb* | ≤5 kb | ≤5 kb |
| Amplicon overhang | Blunt | 3′A | Blunt |
| Inhibitor tolerance | No | ||
| DNA synthesis rate | 15–30 sec/kb | 15 sec/kb | 60 sec/kb |
| Universal primer annealing | No | ||
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | ≤1 copy (per 50 μL rxn) | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | One optimized buffer (GC enhancer supplied in a separate vial) | Two-buffer system (based on GC%) |
| Product formats | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless |
* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading
| Platinum SuperFi II DNA Polymerase | AccuPrime Taq DNA Polymerase, High Fidelity | AccuPrime Pfx DNA Polymerase | |
|---|---|---|---|
| Fidelity (vs. Taq) | >300x | 9x | 26x |
| Hot-start modification | |||
| Universal primer annealing | No | No | |
| Amplification range | 20 kb* | ≤20 kb (with Buffer II) | ≤12 kb |
| Amplicon overhang | Blunt | 3′A and blunt | Blunt |
| GC-rich format | No | No | |
| Inhibitor tolerance | No | No | |
| DNA synthesis rate | 15–30 sec/kb | 60 sec/kb | 60 sec/kb |
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | Not tested | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | Two buffers (based on template type) | One buffer |
| Product formats | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless | Stand alone: Colorless |
* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading
| Platinum II Taq DNA Polymerase | AccuPrime Taq DNA Polymerase System | |
|---|---|---|
| Hot-start modification | ||
| Fidelity (vs. Taq) | 1x | 2x |
| Amplification range | ≤5 kb | ≤5 kb |
| Inhibitor tolerance | No | |
| DNA synthesis rate | 15 sec/kb | 60 sec/kb |
| Amplicon overhang | 3′A | 3′A |
| Universal primer annealing | No | |
| Residual bacterial gDNA | ≤1 copy/enzyme unit | Not tested |
| GC-rich format | No | |
| Multiplexing | Up to 15 | Up to 20 |
| Buffer system | One optimized buffer (GC enhancer supplied in a separate vial) | Two buffers (based on template type) |
| Product formats | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless |
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading
| Platinum SuperFi II DNA Polymerase | Platinum II Taq DNA Polymerase | AccuPrime GC-Rich DNA Polymerase | |
|---|---|---|---|
| GC-rich amplification | >65% GC | >65% GC | >65% GC |
| Hot-start modification | No | ||
| Fidelity (vs. Taq) | >300x | 1x | 2x |
| Amplification range | 20 kb* | ≤5 kb | ≤5 kb |
| Amplicon overhang | Blunt | 3′A | Blunt |
| Inhibitor tolerance | No | ||
| DNA synthesis rate | 15–30 sec/kb | 15 sec/kb | 60 sec/kb |
| Universal primer annealing | No | ||
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | ≤1 copy (per 50 μL rxn) | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | One optimized buffer (GC enhancer supplied in a separate vial) | Two-buffer system (based on GC%) |
| Product formats | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless Master mix: Colorless Green† | Stand alone: Colorless |
* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading
AccuPrime DNA polymerase products
The following table summarizes AccuPrime DNA polymerases and formats available for PCR.
| AccuPrime Taq DNA Polymerase, High Fidelity | AccuPrime Pfx DNA Polymerase | AccuPrime GC-Rich DNA Polymerase | AccuPrime Taq DNA Polymerase System | |
|---|---|---|---|---|
| DNA polymerase(s) | Blend of Taq DNA polymerase and proofreading GB-D enzyme from Pyrococcus species | Recombinant KOD enzyme from Thermococcus species | DNA polymerase cloned from Pyrolobus fumarius | AccuPrime Taq DNA polymerase |
| Hot-start modification | No | |||
| Enhanced specificity with AccuPrime accessory proteins | ||||
| Fidelity (vs. Taq) | 9x | 26x | 2x | 2x |
| Amplicon overhang | 3′A and blunt | Blunt | Blunt | 3′A |
| Product formats | Stand-alone enzyme with the two-buffer system: Buffer I for plasmids, cDNA, and λ DNA; Buffer II for gDNA. | Stand-alone enzyme | Stand-alone enzyme with the two-buffer system: Buffer A for GC-rich gDNA targets; Buffer B for non–GC-rich gDNA, cDNA, plasmids, and λ DNA. | Stand-alone enzyme with the two-buffer system: Buffer I for small gDNA amplicons (≤200 bp), cDNA, and plasmids; Buffer II for gDNA (200 bp–4 kb). |
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