Protein Expression Resource List | Thermo Fisher Scientific

PG2152-PJT6527-COL112335-ET4-Protein-Expression-Resource-List-Global-F

Learn about methods and technologies for cell-based or cell-free protein production, including algal, bacterial, mammalian, insect, and yeast expression systems; and vector systems that are easy to use and deliver high protein yields for every downstream application.

Recombinant protein production
Host & vector systems
Cell-based vs. cell-free expression
Transformation
Cell culture
Technical handbooks

Protein methods articles
Product application notes
Scientific posters
Support center resources
Protocols
Product selection guides

Protein expression features

Handbook

Protein Expression Handbook

This 118-page handbook provides comprehensive information about protein expression and will help you choose the right expression system and purification technologies for your specific application and needs.

Educational Hub

Gibco Protein Expression Basics

Gibco Protein Expression Basics is an online hub that brings together educational content and tools to enhance your research. Here you can access our protein expression selection guide, educational videos, and other technical resources. Start here if you are new to protein expression or need to refresh your knowledge.

Support center

Protein Expression Support Center

One resource for all your protein expression support needs. Obtain relevant technical information, view tips and tricks when starting an experiment, and find answers to everyday problems related to protein expression.

Publications

Protein Expression Publication Hub

The Protein Expression Publication Hub features a curated list of publications that offers an in-depth look into how scientists like you advance their research with Expi protein expression systems. Discover how you can achieve high-yield, consistent proteins for a diverse array of downstream applications today.

Protein expression learning resources

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Type	Title
Handbook	Protein Research Handbook (2016)
Handbook	Protein Expression Handbook (2015)
Literature	Gibco Expi protein expression systems brochure (Expi293 and ExpiCHO)
Literature	ExpiSf expression system flyer
Literature	Expi293 tools for structural biology and inducible expression
Methods article	The Pierce Protein Methods Library
Methods article	GST-tagged Proteins–Production and Purification
Methods article	His-tagged Proteins–Production and Purification
Methods article	Luciferase Reporters
Methods article	An Overview of Post-Translational Modification
Methods article	Phosphorylation
Methods article	Glycosylation
Methods article	An Overview of Protein Expression Systems
Methods article	Cell-Free Protein Expression
Selection guide	Protein Expression
Selection guide	Luciferase Detection Instrumentation
Selection guide	Luciferase Reporter Selection Guide
Selection guide	Overview of Human in vitro Translation Systems
Selection guide	Protein Expression Essentials
Selection guide	Protein Expression Host Systems
Application note	Scalable production and purification of adeno-associated virus (AAV) vector using the ExpiSf Expression System
Application note	Adaptation of the ExpiSf Expression System for high-level protein production with the WAVE bioreactor system
Application note	ExpiCHO Expression System—Protocols for 24 and 96 deep well blocks and mini bioreactor tubes
Application note	ExpiCHO Expression System: A guide to optimizing protein A purification of monoclonal antibodies
Application note	Adaptation of Expi293 to the WAVE Bioreactor System
Application note	Expi293 MembranePro Expression System App Note
Application note	The Expi293 Expression System—Optimizing protein yield
Application note	Expi293 Expression System—Optimizing culture conditions to maximize protein yield and enable use of 96-well microtiter plates
Application note	Using the Expi293 Expression System under animal origin–free (AOF) conditions
Application note	Product Application Notes
Application note	Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System.
Application note	Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria.
Application note	System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates.
Application note	Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system.
Application note	Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates.
Application note	Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader.
Application note	Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry.
Application note	Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays.
Scientific poster	Scalable protein and AAV production in the ExpiSf Expression System
Scientific poster	ExpiCHO: Surpassing the Performance of Expi293 in a Transient CHO Expression System
Scientific poster	Optimized expression of membrane proteins in the Expi293 and ExpiCHO expression systems: new tools for difficult to express proteins
Scientific poster	Strategies for high-titer protein expression using the ExpiCHO and Expi293 transient expression systems
Scientific poster	ExpiSf expression system: a chemically defined baculovirus-based system for enhanced protein and virus production in Sf9 cells
Support center	Protein Expression Support Center
Support center	Adenoviral Gene Delivery for Mammalian Expression Support
Support center	Algae Expression Support
Support center	Bacterial Expression Support
Support center	Basic Mammalian Expression Support
Support center	Cell-Free Expression Support
Support center	Insect Expression Support
Support center	Lentiviral Gene Delivery for Mammalian Expression Support
Support center	Mammalian Expression for High-Yield Protein Production Support
Support center	Mammalian Expression for Membrane Protein Production Support
Support center	Protein Expression Basics Support
Support center	Reporter & Specialty Vectors for Mammalian Expression Support
Support center	Yeast Expression Support
Protocol	ExpiCHO essential protocol guide
Protocol	Expi293 expression system user guide
Protocol	ExpiSf smart start guide
Protocol	Protein Expression Protocols
Protocol calculator	Expi Systems Protocol Calculator
Webinar	Enabling Next-Gen Targeted siRNA with ExpiCHO
Webinar	Expression, purification and characterization in Expi293 and ExpiCHO
Webinar	Factor V Expression in ExpiCHO Cells
Webinar	Expi293: New Tools for structural Biology and difficult proteins
Webinar	ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Webinar	ExpressionWorld Webcasts

Protein Biology Resource Library

Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.

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Resources

Support

For Research Use Only. Not for use in diagnostic procedures.