Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Biotin, eBioscience™

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 19

CD90.2 (Thy-1.2) Antibody (13-0902-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD90.2 (Thy-1.2) FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

13-0902-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 22 publications 22 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-2.1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466533

Product Specific Information

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury.

Nature communications

Bi R,Yin Q,Li H,Yang X,Wang Y,Li Q,Fang H,Li P,Lyu P,Fan Y,Ying B,Zhu S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis.

Nature communications

Beckmann D,Römer-Hillmann A,Krause A,Hansen U,Wehmeyer C,Intemann J,de Gorter DJJ,Dankbar B,Hillen J,Heitzmann M,Begemann I,Galic M,Weinhage T,Foell D,Ai R,Kremerskothen J,Kiener HP,Müller S,Kamradt T,Schröder C,Leitão E,Horsthemke B,Rosenstiel P,Nordström K,Gasparoni G,Gasparoni N,Walter J,Li N,Yang X,Chung HR,Pavenstädt H,Lindemann N,Schnittler HJ,Wang W,Firestein GS,Pap T,Korb-Pap A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Immunohistochemistry

Tue Jun 15 00:00:00 EDT 2021

SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms.

Scientific reports

Wang Y,Mohseni M,Grauel A,Diez JE,Guan W,Liang S,Choi JE,Pu M,Chen D,Laszewski T,Schwartz S,Gu J,Mansur L,Burks T,Brodeur L,Velazquez R,Kovats S,Pant B,Buruzula G,Deng E,Chen JT,Sari-Sarraf F,Dornelas C,Varadarajan M,Yu H,Liu C,Lim J,Hao HX,Jiang X,Malamas A,LaMarche MJ,Geyer FC,McLaughlin M,Costa C,Wagner J,Ruddy D,Jayaraman P,Kirkpatrick ND,Zhang P,Iartchouk O,Aardalen K,Cremasco V,Dranoff G,Engelman JA,Silver S,Wang H,Hastings WD,Goldoni S

13-0902-82 was used in Flow Cytometry to reveal novel functions of SHP2 in tumor immunity and proposes that targeting SHP2 is a promising strategy for cancer immunotherapy.

Thu Jan 14 00:00:00 EST 2021

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Sun Apr 17 00:00:00 EDT 2022

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Single-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte.

Nature

Liu Z,Wang L,Welch JD,Ma H,Zhou Y,Vaseghi HR,Yu S,Wall JB,Alimohamadi S,Zheng M,Yin C,Shen W,Prins JF,Liu J,Qian L

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Immunofluorescence

Thu Nov 02 00:00:00 EDT 2017

Non-coding Transcription Instructs Chromatin Folding and Compartmentalization to Dictate Enhancer-Promoter Communication and T Cell Fate.

Cell

Isoda T,Moore AJ,He Z,Chandra V,Aida M,Denholtz M,Piet van Hamburg J,Fisch KM,Chang AN,Fahl SP,Wiest DL,Murre C

13-0902-82 was used in Flow Cytometry to study the repositioning of the Bcl11b enhancer to dictate the fate of developing T cells.

Thu Sep 21 00:00:00 EDT 2017

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

The composition of the microbiota modulates allograft rejection.

The Journal of clinical investigation

Lei YM,Chen L,Wang Y,Stefka AT,Molinero LL,Theriault B,Aquino-Michaels K,Sivan AS,Nagler CR,Gajewski TF,Chong AS,Bartman C,Alegre ML

13-0902 was used in Magnetic cell separation to test the influence of host and donor microbiota on skin transplant rejection.

Fri Jul 01 00:00:00 EDT 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Distinct germinal center selection at local sites shapes memory B cell response to viral escape.

The Journal of experimental medicine

Adachi Y,Onodera T,Yamada Y,Daio R,Tsuiji M,Inoue T,Kobayashi K,Kurosaki T,Ato M,Takahashi Y

13-0902 was used in Flow cytometry/Cell sorting to investigate the cellular events dictating the cross-reactive memory B cell responses, showing that distinct germinal center selection at local sites shapes response to viral escape.

Mon Sep 21 00:00:00 EDT 2015

Distinct germinal center selection at local sites shapes memory B cell response to viral escape.

The Journal of experimental medicine

Adachi Y,Onodera T,Yamada Y,Daio R,Tsuiji M,Inoue T,Kobayashi K,Kurosaki T,Ato M,Takahashi Y

Mon Sep 21 00:00:00 EDT 2015

Redundant Function of Plasmacytoid and Conventional Dendritic Cells Is Required To Survive a Natural Virus Infection.

Journal of virology

Kaminsky LW,Sei JJ,Parekh NJ,Davies ML,Reider IE,Krouse TE,Norbury CC

13-0902 was used in Flow cytometry/Cell sorting to explore the roles of individual myeloid cell subsets in the survival of ectromelia virus infection.

Thu Oct 01 00:00:00 EDT 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8alpha+ conventional dendritic cells.

The Journal of experimental medicine

Edelson BT,KC W,Juang R,Kohyama M,Benoit LA,Klekotka PA,Moon C,Albring JC,Ise W,Michael DG,Bhattacharya D,Stappenbeck TS,Holtzman MJ,Sung SS,Murphy TL,Hildner K,Murphy KM

13-0902 was used in Flow cytometry/Cell sorting to study the relationship between nonlymphoid CD103-expressing dendritic cells (DCs) and other DC subsets.

Mon Apr 12 00:00:00 EDT 2010

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

13-0902 was used in Flow cytometry/Cell sorting to track epitope-specific T cells.

Tue Jun 30 00:00:00 EDT 2009

The novel role of tyrosine kinase inhibitor in the reversal of immune suppression and modulation of tumor microenvironment for immune-based cancer therapies.

Cancer research

Ozao-Choy J,Ma G,Kao J,Wang GX,Meseck M,Sung M,Schwartz M,Divino CM,Pan PY,Chen SH

13-0902 was used in Flow cytometry/Cell sorting to suggest that sunitinib can be used to reverse immune suppression and as a potentially useful adjunct for enhancing the efficacy of immune-based cancer therapy for advanced malignancies.

Sun Mar 15 00:00:00 EDT 2009

Viral sequestration of antigen subverts cross presentation to CD8(+) T cells.

PLoS pathogens

Tewalt EF,Grant JM,Granger EL,Palmer DC,Heuss ND,Gregerson DS,Restifo NP,Norbury CC

13-0902 was used in Flow cytometry/Cell sorting to describe an evasion mechanism employed by pathogens to prevent entry into the cross-presentation pathway.

Fri May 01 00:00:00 EDT 2009

Manganese superoxide dismutase gene dosage affects chromosomal instability and tumor onset in a mouse model of T cell lymphoma.

Free radical biology & medicine

van de Wetering CI,Coleman MC,Spitz DR,Smith BJ,Knudson CM

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0902-82) in Flow Cytometry

Tue Apr 15 00:00:00 EDT 2008

An MHC-linked locus modulates thymic differentiation of CD4+CD25+Foxp3+ regulatory T lymphocytes.

International immunology

Tellier J,van Meerwijk JP,Romagnoli P

13-0902 was used in Flow cytometry/Cell sorting to investigate factors that affect the levels of Tregs in the thymus.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

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Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831

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Entrez Gene ID: (Mouse) 21838

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding cell adhesion molecule

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Biotin, eBioscience™

CD90.2 (Thy-1.2) Antibody (13-0902-82) in Flow

Product Details

13-0902-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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