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Invitrogen
ATG10 Recombinant Rabbit Monoclonal Antibody (23GB4860)
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Application
FIGURE: 1 / 2
ATG10 Antibody (MA5-52752) in WB
Western Blot analysis of ATG10. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG10 recombinant monoclonal antibody (Product # MA5-52752) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
Product Details
MA5-52752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB4860
Immunogen
A synthesized peptide derived from human Apg10 (Atg10) (170-220AA).
Conjugate
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3249229
Target Information
ATG10 is involved in autophagy which is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12 -ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein ATG10 is ubiquitously expressed and the human APG10L gene is localized on chromosome 5q14.2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG10-like; ATG10 autophagy related 10 homolog; Autophagy-related protein 10; DKFZp586I0418; FLJ13954; Ubiquitin-like-conjugating enzyme ATG10
Gene Aliases: APG10; APG10L; ATG10; PP12616
UniProt ID: (Human) Q9H0Y0
Entrez Gene ID: (Human) 83734
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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