Invitrogen

CD24 Monoclonal Antibody (30-F1), FITC, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (6)

FIGURE: 1 / 7

CD24 Antibody (11-0241-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 APC (Product # 17-0191-82) and 0.5 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (left) or 0.5 µg of Anti-Mouse CD24 FITC (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

11-0241-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

30-F1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_464985

Product Specific Information

Description: The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. The expression of CD24 detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and different antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations.

Applications Reported: The 30-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 30-F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

In Lyl1^-/- mice, adipose stem cell vascular niche impairment leads to premature development of fat tissues.

Stem cells (Dayton, Ohio)

Hussain A,Deleuze V,El Kebriti L,Turali H,Pirot N,Glasson Y,Mathieu D,Pinet V

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

In Lyl1^-/- mice, adipose stem cell vascular niche impairment leads to premature development of fat tissues.

Stem cells (Dayton, Ohio)

Hussain A,Deleuze V,El Kebriti L,Turali H,Pirot N,Glasson Y,Mathieu D,Pinet V

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Gli3 in fetal thymic epithelial cells promotes thymocyte positive selection and differentiation by repression of Shh.

Development (Cambridge, England)

Solanki A,Yanez DC,Ross S,Lau CI,Papaioannou E,Li J,Saldaña JI,Crompton T

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining.

Nature communications

Li C,Irrazabal T,So CC,Berru M,Du L,Lam E,Ling AK,Gommerman JL,Pan-Hammarström Q,Martin A

Thu Mar 08 00:00:00 EST 2018

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Lineage-Specific Genes Are Prominent DNA Damage Hotspots during Leukemic Transformation of B Cell Precursors.

Cell reports

Boulianne B,Robinson ME,May PC,Castellano L,Blighe K,Thomas J,Reid A,Müschen M,Apperley JF,Stebbing J,Feldhahn N

11-0241 was used in Flow cytometry/Cell sorting to demonstrate lineage-specific transcription predisposes genes in B cells to DNA damage.

Tue Feb 14 00:00:00 EST 2017

β-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the Reticular Dermis.

The Journal of investigative dermatology

Mastrogiannaki M,Lichtenberger BM,Reimer A,Collins CA,Driskell RR,Watt FM

11-0241 was used in Flow cytometry/Cell sorting to study how Wnt/β-catenin signalling may control adipogenic cell fate within the lower dermis, which potentially contributes to the pathogenesis of fibrotic skin diseases.

Wed Jun 01 00:00:00 EDT 2016

Enterocolitis causes profound lymphoid depletion in endothelin receptor B- and endothelin 3-null mouse models of Hirschsprung-associated enterocolitis.

European journal of immunology

Frykman PK,Cheng Z,Wang X,Dhall D

11-0241 was used in Flow cytometry/Cell sorting to investigate how enterocolitis in the Ednrb(-/-) and Edn3(-/-) mice lead to thymic involution, splenic lymphopenia, and suppression of B lymphopoiesis as a consequence of colonic aganglionosis.

Sun Mar 01 00:00:00 EST 2015

Processing of CD74 by the Intramembrane Protease SPPL2a Is Critical for B Cell Receptor Signaling in Transitional B Cells.

Journal of immunology (Baltimore, Md. : 1950)

Hüttl S,Kläsener K,Schweizer M,Schneppenheim J,Oberg HH,Kabelitz D,Reth M,Saftig P,Schröder B

11-0241 was used in Flow cytometry/Cell sorting to demonstrate that tonic as well as BCR-induced activation of the PI3K/Akt pathway is massively compromised in SPPL2a(-/-) B cells.

Sat Aug 15 00:00:00 EDT 2015

Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.

Cell cycle (Georgetown, Tex.)

Qin Y,Qin J,Zhou C,Li J,Gao WQ

11-0241 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning.

Tue Jan 26 00:00:00 EST 2016

Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.

Cancer research

Lee CY,Lin Y,Bratman SV,Feng W,Kuo AH,Scheeren FA,Engreitz JM,Varma S,West RB,Diehn M

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD24 monoclonal antibody (Product # 11-0241-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Effects of HCV on basal and tat-induced HIV LTR activation.

PloS one

Sengupta S,Powell E,Kong L,Blackard JT

11-0241 was used in Flow cytometry/Cell sorting to evaluate the effects of HCV on basal and Tat-induced activation of the HIV long terminal repeat in hepatocytes.

Tue Feb 11 00:00:00 EST 2014

ZBTB1 is a determinant of lymphoid development.

The Journal of experimental medicine

Siggs OM,Li X,Xia Y,Beutler B

11-0241 was used in Flow cytometry/Cell sorting to identify a mutation causative of complete and cell-intrinsic T cell deficiency.

Mon Jan 16 00:00:00 EST 2012

Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Smith KA,Hochweller K,Hämmerling GJ,Boon L,MacDonald AS,Maizels RM

11-0241 was used in Flow cytometry/Cell sorting to identify that the preferential expansion of a novel population of CD11c(lo) non-plasmacytoid DCs is associated with the pro-regulatory environment of chronic intestinal helminth infection.

Wed Jun 15 00:00:00 EDT 2011

c-Myb is required for pro-B cell differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Fahl SP,Crittenden RB,Allman D,Bender TP

11-0241 was used in Flow cytometry/Cell sorting to demonstrate that c-Myb is absolutely required for the survival and differentiation of CD19(+) B-lineage cells.

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen

View more View less

Gene Aliases: Cd24; Cd24a; HSA; Ly-52; nectadrin

View more View less

UniProt ID: (Mouse) P24807

View more View less

Entrez Gene ID: (Mouse) 12484

View more View less

Function(s)

protein binding protein kinase binding carbohydrate binding protein tyrosine kinase activator activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD24 Monoclonal Antibody (30-F1), FITC, eBioscience™

CD24 Antibody (11-0241-82) in Flow

Product Details

11-0241-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!