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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • CD45 Antibodies

            Invitrogen

            CD45 Monoclonal Antibody (30-F11), FITC, eBioscience™

            98个已发表图片
            436 篇参考文献
            查看其他313个CD45抗体
            说明书
            实验方案
            问题与答案
            技术支持
            货号 11-0451-82
            Web quote
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            Price (CNY)
            652.00
            飞享价
            Ends: 31-Dec-2025
            872.00
            Save 220.00 (25%)
             
            有货
            查看价格
            批量或定制请求
            说明书
            实验方案
            问题与答案
            技术支持

            Cite CD45 Monoclonal Antibody (30-F11), FITC, eBioscience™

            • 抗体检测数据 (1)
            • 已发表图片 (98)
            Application
            CD45 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            图: 1 / 99

            CD45 Antibody (11-0451-82) in Flow

            C57BL/6 mouse splenocytes were stained with TER-119 Monoclonal Antibody, APC (Product # 17-5921-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, FITC (Product # 11-4031-85) (left) or 0.125 µg of CD45 Monoclonal Antibody, FITC (right). Cells in the lymphocyte gate were used for analysis.
            查看产品

            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)

            产品信息

            11-0451-82

            应用
            建议稀释比
            已发表文章

            免疫印迹 (WB)

            -
            查看1篇已发表文章 1篇已发表文章

            免疫组化 (IHC)

            -
            查看41篇已发表文章 41篇已发表文章

            免疫组化(石蜡) (IHC (P))

            -
            查看1篇已发表文章 1篇已发表文章

            免疫组织化学(多聚甲醛固定) (IHC (PFA))

            -
            查看1篇已发表文章 1篇已发表文章

            免疫组化(冰冻) (IHC (F))

            -
            查看6篇已发表文章 6篇已发表文章

            免疫细胞化学 (ICC/IF)

            -
            查看10篇已发表文章 10篇已发表文章

            流式细胞分析 (Flow)

            0.5 µg/test
            查看375篇已发表文章 375篇已发表文章

            其他PubMed (Misc)

            -
            查看1篇已发表文章 1篇已发表文章
            产品规格

            种属反应

            Mouse

            已发表种属

            Horse, Human, Mouse, Rat

            宿主/亚型

            Rat / IgG2b, kappa

            推荐的同型对照

            Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

            分类

            Monoclonal

            类型

            Antibody

            克隆号

            30-F11

            偶联物

            FITC
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 532 
            • Alexa Fluor 561
            • Alexa Fluor 660 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 450
            • eFluor 506
            • Functional Grade
            • NovaFluor Blue 510
            • NovaFluor Blue 555
            • NovaFluor Blue 585
            • NovaFluor Blue 610-30S
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 660-40S
            • NovaFluor Blue 725
            • NovaFluor Blue 760
            • NovaFluor Blue 800
            • NovaFluor Red 700
            • NovaFluor Red 710
            • NovaFluor UV 765
            • NovaFluor Violet 690
            • NovaFluor Violet 800
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • NovaFluor Yellow 690
            • NovaFluor Yellow 700
            • NovaFluor Yellow 730
            • NovaFluor Yellow 810
            • PE 
            • PE-Cyanine5
            • PE-Cyanine5.5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            激发/发射光谱

            498/517 nm 查看光谱 spectra

            形式

            Liquid

            浓度

            0.5 mg/mL

            纯化类型

            Affinity chromatography

            保存液

            PBS, pH 7.2

            内含物

            0.09% sodium azide

            保存条件

            4°C, store in dark, DO NOT FREEZE!

            运输条件

            Ambient (domestic); Wet ice (international)

            RRID

            AB_465050

            产品详细信息

            Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

            Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            靶标信息

            CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

            仅用于科研。不用于诊断过程。未经明确授权不得转售。

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            生物信息学

            蛋白别名: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

            查看更多 收起

            基因别名: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

            查看更多 收起

            UniProt ID: (Mouse) P06800

            查看更多 收起

            Entrez Gene ID: (Mouse) 19264

            查看更多 收起

            功能

            phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

            参与通路

            activation of MAPK activity negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion response to gamma radiation dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation T cell proliferation B cell proliferation positive regulation of T cell proliferation positive regulation of MAPK cascade cell cycle phase transition positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development negative regulation of peptidyl-tyrosine phosphorylation T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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