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Application
FIGURE: 1 / 1
FIG4 Antibody (PA5-120353) in WB
Western blot analysis of FIG4 in extracts of various cell lines. Samples were incubated with FIG4 Polyclonal antibody (Product # PA5-120353) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-120353
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 608-907 of human FIG4 (NP_055660.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.29 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913925
Product Specific Information
Positive test controls include: U-251MG, HT-29, Jukat, HeLa, Mouse liver, Mouse spleen. The target is usually found in the following locations: Endosome membrane.
Immunogen sequence: WELPTDFYLH HKNTMRLLPT RRSYTYWWTP EVIKHLPLPY DEVICAVNLK KLIVKKFHKY EEEIDIHNEF FRPYELSSFD DTFCLAMTSS ARDFMPKTVG IDPSPFTVRK PDETGKSVLG NKSNREEAVL QRKTAASAPP PPSEEAVSSS SEDDSGTDRE EEGSVSQRST PVKMTDAGDS AKVTENVVQP MKELYGINLS DGLSEEDFSI YSRFVQLGQS QHKQDKNSQQ PCSRCSDGVI KLTPISAFSQ DNIYEVQPPR VDRKSTEIFQ AHIQASQGIM QPLGKEDSSM YREYIRNRYL
Target Information
The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FIG4 homolog, SAC domain containing lipid phosphatase; FIG4 homolog, SAC1 domain containing lipid phosphatase; FIG4 homolog, SAC1 lipid phosphatase domain containing; Phosphatidylinositol 3,5-bisphosphate 5-phosphatase; Polyphosphoinositide phosphatase; RP1-249I4.1; Sac domain-containing inositol phosphatase 3; SAC domain-containing protein 3; Serine-protein phosphatase FIG4
Gene Aliases: A530089I17Rik; AI326867; ALS11; BTOP; CMT4J; dJ249I4.1; FIG4; KIAA0274; SAC3; YVS
UniProt ID: (Human) Q92562, (Mouse) Q91WF7
Entrez Gene ID: (Human) 9896, (Mouse) 103199
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