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Application
FIGURE: 1 / 2
GEF-H1 Antibody (720323) in ICC/IF
For immunofluorescence analysis, K562 cells were fixed and permeabilized for detection of endogenous GEF-H1 using Anti- GEF-H1 Rabbit Polyclonal Antibody (Product # 720323, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of GEF-H1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel... View More
Product Details
720323
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human ARHGEF2 (aa 20-37, 969-986)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633257
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Bovine and Horse
Target Information
GEF-H1 activates Rho GTPases by promoting the exchange of GDP and GTP. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. GEF-H1 may be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp547L106; DKFZp547P1516; FLJ45440; GEF-H1; Guanine nucleotide exchange factor H1; LBC'S first cousin; Lymphoid blast crisis-like 1; Microtubule-regulated Rho-GEF; Oncogene LFC; OTTHUMP00000018855; Proliferating cell nucleolar antigen p40; Rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rhobin
Gene Aliases: AA408978; ARHGEF2; GEF; GEF-H1; GEFH1; KIAA0651; Lbcl1; Lfc; LFP40; mKIAA0651; P40
UniProt ID: (Human) Q92974, (Mouse) Q60875
Entrez Gene ID: (Human) 9181, (Mouse) 16800
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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