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Invitrogen

Human Hematopoietic Lineage Antibody Cocktail, APC, eBioscience™

5 References

View all (5) Hematopoietic Lineage antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)

Application

Human Hematopoietic Lineage Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Human Hematopoietic Lineage Antibody (22-7776-72) in Flow

Normal human peripheral blood cells were stained with Anti-Human CD34 PerCP-eFluor® 710 (Product # 46-0349-42), Anti-Human CD133 PE and the Human Hematopoietic Lineage APC Cocktail. Cells in the lymphocyte gate were used for analysis. Cells in the right dot plot are gated on lineage negative events, depicted in the left histogram.

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Product Details

22-7776-72

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

20 µL/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Cocktail

Type

Antibody

Clone

RPA-2.10, OKT3, 61D3, CB16, HIB19, TULY56, HIR2

Conjugate

APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2043865

Product Specific Information

Description: The Human Hematopoietic Lineage APC Cocktail contains APC-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

This cocktail contains the following antibodies:

Anti-Human CD2 (RPA-2.10) APC

Anti-Human CD3 (OKT3) APC

Anti-Human CD14 (61D3) APC

Anti-Human CD16 (CB16) APC

Anti-Human CD19 (HIB19) APC

Anti-Human CD56 (TULY56) APC

Anti-Human CD235a (HIR2) APC

Applications Reported: The Human Hematopoietic Lineage APC Cocktail has been reported for use in flow cytometric analysis.

Applications Tested: The Human Hematopoietic Lineage APC Cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Target Information

Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Characterizing diversity among human oral stem-like cancer cells using flow cytometry.

STAR protocols

Ghosh S,Mitra P,Saha U,Vipparthi K,Singh S

22-7776-72 was used in Flow Cytometry to develop the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors.

Fri Dec 16 00:00:00 UTC 2022

Emergence of hybrid states of stem-like cancer cells correlates with poor prognosis in oral cancer.

iScience

Vipparthi K,Hari K,Chakraborty P,Ghosh S,Patel AK,Ghosh A,Biswas NK,Sharan R,Arun P,Jolly MK,Singh S

22-7776-72 was used in Flow cytometry/Cell sorting to describe the phenotypic-composition of heterogeneous subpopulations critical for global tumor behavior in oral cancer; which may provide prerequisite knowledge for treatment strategies.

Fri May 20 00:00:00 UTC 2022

Transitions in lineage specification and gene regulatory networks in hematopoietic stem/progenitor cells over human development.

Cell reports

Roy A,Wang G,Iskander D,O'Byrne S,Elliott N,O'Sullivan J,Buck G,Heuston EF,Wen WX,Meira AR,Hua P,Karadimitris A,Mead AJ,Bodine DM,Roberts I,Psaila B,Thongjuea S

22-7776-72 was used in Flow cytometry/Cell sorting to demonstrate the utility of this dataset for understanding aberrant hematopoiesis through comparison to two cancers that present at distinct time points in postnatal life-juvenile myelomonocytic leukemia, a childhood cancer, and myelofibrosis, which classically presents in older adults.

Tue Sep 14 00:00:00 UTC 2021

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

22-7776 was used in Flow cytometry/Cell sorting to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 UTC 2017

Junctional Adhesion Molecule-A Is Highly Expressed on Human Hematopoietic Repopulating Cells and Associates with the Key Hematopoietic Chemokine Receptor CXCR4.

Stem cells (Dayton, Ohio)

Chang CH,Hale SJ,Cox CV,Blair A,Kronsteiner B,Grabowska R,Zhang Y,Cook D,Khoo CP,Schrader JB,Kabuga SB,Martin-Rendon E,Watt SM

22777672 was used in flow cytometry to explore how junctional adhesion molecule family members differentially regulate CXCR4 function and CXCL12 secretion in the bone marrow niche

Wed Jun 01 00:00:00 UTC 2016

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