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Invitrogen
IBA1 Recombinant Rabbit Monoclonal Antibody (145)
Application
FIGURE: 1 / 5
IBA1 Antibody (MA5-29013) in WB
Western blot was performed using Anti-IBA1 Recombinant Rabbit Monoclonal Antibody (145) (Product # MA5-29013) and a 16 kDa band corresponding to Allograft inflammatory factor 1 was observed across positive cell lines (THP-1 and HL-60) and positive tissues (Mouse spleen and Mouse thymus tested) tested. Also, a band was observed in U937 treated with Retinoic acid (10 uM, 24 hours) treatment, showing upregulation of IBA1. Membrane enriched extracts (30 µg lysate) of U-937 (Lane 1), U-937 treated with Retinoic acid (Lane 2), HL-60 (Lane 3), TH... View More
Product Details
MA5-29013
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
145
Immunogen
Recombinant Human IBA1/AIF1 Protein (Met1-Pro147)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784951
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human IBA1/AIF1.
Target Information
Ionized calcium-binding adapter molecule 1 (IBA1), also known by its gene name AIF1, is a protein expressed predominantly by microglia in the brain and spinal cord. This protein belongs to the EF-hand calcium-binding protein family and plays a crucial role in microglial activation and migration in response to brain injury or neuroinflammation. IBA1's function is integral to microglial motility and phagocytic activity, facilitating the cellular response to pathogenic stimuli and promoting tissue homeostasis and repair in the central nervous system. IBA1 serves as a reliable marker for activated microglia in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and multiple sclerosis, where increased expression correlates with disease progression and severity. The protein's structural features enable it to bind calcium ions, inducing conformational changes that activate signaling pathways essential for microglial function. Its expression is highly regulated by inflammatory cytokines, underpinning its role in neuroimmune responses. Due to its specific expression in microglia during pathological conditions, IBA1 is widely used in research as a marker to study microglial status and activity, and it remains a focal point for understanding microglial involvement in neurodegenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIF-1; Allograft inflammatory factor 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Protein G1; testis specific
Gene Aliases: AI607846; AIF-1; AIF1; D17H6S50E; G1; IBA1; IRT-1; IRT1
UniProt ID: (Human) P55008, (Mouse) O70200
Entrez Gene ID: (Human) 199, (Mouse) 11629
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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