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            • Primary Antibodies ›
            • Ki-67 Antibodies

            Invitrogen

            Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience™

            Advanced Verification
            93 Published Figures
            237 References
            View all (222) Ki-67 antibodies

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            Datasheet
            Protocols
            Questions & Answers
            Catalog # 11-5698-82
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            Price (USD)
            488.00
             
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            Cite Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience™

            • Antibody Testing Data (2)
            • Published Figures (93)
            • Advanced Verification (1)
            Application
            Ki-67 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 96

            Ki-67 Antibody (11-5698-82) in Flow

            C57Bl/6 splenocytes stimulated for 2 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) were surface stained with Anti-Mouse CD19 PE (Product # 12-0193-82). Cells were then fixed and permeabilized using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and stained intracellularly with 0.125 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse/Rat Ki-67 FITC (right).
            View Product

            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            Ki-67 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            Ki-67 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Western Blot, Immunohistochemistry, Flow Cytometry (WB, IHC, Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Flow Cytometry (Flow)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))
            Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody in Immunocytochemistry (ICC/IF)
            Ki-67 Antibody

            Product Details

            11-5698-82

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            -
            View 1 publication 1 publication

            Immunohistochemistry (IHC)

            Assay-Dependent
            View 70 publications 70 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            -
            View 3 publications 3 publications

            Immunohistochemistry (PFA fixed) (IHC (PFA))

            -
            View 2 publications 2 publications

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            10 µg/mL
            View 45 publications 45 publications

            Flow Cytometry (Flow)

            0.25 µg/test
            View 113 publications 113 publications

            Functional Assay (Functional)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat

            Published species

            Guinea pig, Human, Mouse, Rat

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            SolA15

            Conjugate

            FITC
            • Unconjugated
            • Alexa Fluor 488 
            • Alexa Fluor 532 
            • Alexa Fluor 660 
            • Alexa Fluor 700 
            • Alexa Fluor Plus 594 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 450
            • eFluor 506
            • eFluor 570
            • eFluor 615
            • eFluor 660
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-eFluor 710
            • Request custom conjugation

            Excitation/Emission Max

            498/517 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_11151330

            Product Specific Information

            Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

            The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

            Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

            Applications Tested: This SolA15 antibody has been tested by immunocytochemistry on fixed and permeabilized OLN93 cells at less than or equal to 10 µg/mL. It has also been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. For flow application this can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Flow Cytometry
            • Additional Formats
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            Bioinformatics

            Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product

            View more View less

            Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

            View more View less

            UniProt ID: (Human) P46013, (Mouse) E9PVX6

            View more View less

            Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus monkey) 102135895, (Rat) 291234, (Mouse) 17345

            View more View less

            Function(s)

            protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function

            Process(es)

            DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation
            It has to be done as per old AB suggested Products section.
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