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            • Primary Antibodies ›
            • OR2T4 Antibodies

            Invitrogen

            OR2T4 Polyclonal Antibody

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            Cite OR2T4 Polyclonal Antibody

            • Antibody Testing Data (3)
            Application
            OR2T4 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            OR2T4 Antibody (PA5-99916) in ICC/IF

            Immunocytochemistry analysis of OR2T4 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with OR2T4 polyclonal antibody (Product # PA5-99916) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
            View Product

            OR2T4 Antibody in Immunocytochemistry (ICC/IF)
            OR2T4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            OR2T4 Antibody in Western Blot (WB)

            Product Details

            PA5-99916

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000-1:3,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:200
            -

            Immunocytochemistry (ICC/IF)

            1:100-1:500
            -
            Product Specifications

            Species Reactivity

            Human, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A synthesized peptide derived from human OR2T4(Accession Q8NH00), corresponding to amino acid residues S178-E228.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.4, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2818849

            Product Specific Information

            Antibody detects endogenous levels of total OR2T4.

            Target Information

            Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: Olfactory receptor 2T4; Olfactory receptor OR1-60; olfactory receptor, family 2, subfamily T, member 4

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            Gene Aliases: OR1-60; OR2T4; OR2T4Q

            View more View less

            UniProt ID: (Human) Q8NH00

            View more View less

            Entrez Gene ID: (Human) 127074

            View more View less

            Function(s)

            G-protein coupled receptor activity olfactory receptor activity transmembrane signal receptor

            Process(es)

            G-protein coupled receptor signaling pathway sensory perception of smell detection of chemical stimulus involved in sensory perception of smell
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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