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            • Primary Antibodies ›
            • RNF2 Antibodies

            Rockland

            RING1B Polyclonal Antibody

            View all (21) RNF2 antibodies
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            Cite RING1B Polyclonal Antibody

            • Antibody Testing Data (2)
            Application
            RING1B Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            RING1B Antibody (600-101-292) in ICC/IF

            Immunofluorescence Microscopy of Goat anti-RING1B antibody. Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear cou... View More
            View Product

            RING1B Antibody in Immunocytochemistry (ICC/IF)
            RING1B Antibody in Western Blot (WB)

            Product Details

            600-101-292

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:5,000-1:10,000
            -

            Immunocytochemistry (ICC/IF)

            1:300
            -

            ELISA (ELISA)

            1:5,000-1:25,000
            -

            Immunoprecipitation (IP)

            1:500-1:2,000
            -
            Product Specifications

            Species Reactivity

            Bovine, Dog, Chicken, Chimpanzee, Human, Mouse, Non-human primate, Rat, Xenopus

            Host/Isotype

            Goat / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1.1 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

            Contains

            0.01% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

            Affinity purified Anti-RING1B antibody is directed against human RING1B protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.

            Target Information

            RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: BAP 1; DinG protein; E3 ubiquitin-protein ligase RING2; GS1-120K12.1; HIP2-interacting protein 3; HIPI 3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; Protein DinG; RING 1B; RING 2; RING finger protein 1B; RING finger protein 2; RING finger protein BAP-1; RING-type E3 ubiquitin transferase RING2; RING1b; RNF 2

            View more View less

            Gene Aliases: AI326319; AI450156; AU019207; BAP-1; BAP1; DING; HIPI3; RING1B; RING2; RNF2

            View more View less

            UniProt ID: (Human) Q99496, (Mouse) Q9CQJ4

            View more View less

            Entrez Gene ID: (Human) 6045, (Bovine) 540090, (Dog) 480042, (Chicken) 424452, (Rat) 304850, (Mouse) 19821

            View more View less

            Function(s)

            chromatin binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin protein ligase activity RING-like zinc finger domain binding molecular_function UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter mitotic cell cycle gastrulation with mouth forming second transcription, DNA-templated germ cell development anterior/posterior axis specification protein sumoylation spinal cord development response to retinoic acid histone H2A monoubiquitination histone H2A-K119 monoubiquitination negative regulation of sequence-specific DNA binding transcription factor activity histone ubiquitination regulation of transcription, DNA-templated
            It has to be done as per old AB suggested Products section.
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