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Invitrogen
Sarcomeric alpha Actin Monoclonal Antibody (5C5)
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Application
FIGURE: 1 / 3
Sarcomeric alpha Actin Antibody (MA1-26928) in ICC/IF
Immunofluorescent analysis of Sarcomeric alpha actin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde... View More
Product Details
MA1-26928
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit
Published species
Dog,
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
5C5
Immunogen
Purified rabbit striated muscle actin.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_795757
Product Specific Information
This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. It does not react with smooth muscle tissue.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: act; actg; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; Alpha-actin-1; Alpha-cardiac actin; alpha-sma; beta-actin; cytoplasmic beta-actin; dfna20; dfna26; MGC75587; nemaline myopathy type 3; unnamed protein product
Gene Aliases: ACTA; ACTA1; ACTC; ACTC1; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; LVNC4; MPFD; NEM1; NEM2; NEM3; SHPM
UniProt ID: (Human) P68133, (Human) P68032
Entrez Gene ID: (Human) 58, (Rabbit) 100009506, (Human) 70, (Rabbit) 100340339
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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