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            • Primary Antibodies ›
            • TIPIN Antibodies

            Bethyl Laboratories

            TIPIN Polyclonal Antibody

            View all (17) TIPIN antibodies
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            Cite TIPIN Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            TIPIN Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            TIPIN Antibody (A301-474A) in WB

            Detection of human TIPIN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TIPIN antibody (Product # A301-474A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. TIPIN was also immunoprecipitated by rabbit anti-TIPIN antibody A301-473A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
            View Product

            TIPIN Antibody in Western Blot (WB)

            Product Details

            A301-474A

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:2,000-1:10,000
            -

            Immunoprecipitation (IP)

            2-5 µg/mg lysate
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Region between residue 251 and 301 of Human TIMELESS interacting protein.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.20 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            TBS, pH 7.0 to 8.0, with 0.1% BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            The recommended shelf life for this product is 1 year from date of receipt.

            Target Information

            Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: CSM3 homolog; FLJ20516; TIMELESS-interacting protein

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            Gene Aliases: TIPIN

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            UniProt ID: (Human) Q9BVW5

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            Entrez Gene ID: (Human) 54962

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            Function(s)

            protein binding DNA metabolism protein

            Process(es)

            DNA replication checkpoint DNA replication mitotic nuclear division positive regulation of cell proliferation response to UV intra-S DNA damage checkpoint regulation of nuclear cell cycle DNA replication cell cycle phase transition replication fork protection cell division
            It has to be done as per old AB suggested Products section.
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