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Single-Use Fermentors
Designed specifically for microbial fermentation
The Thermo Scientific HyPerforma Single-Use Fermentor (S.U.F.) is pioneering the single-use technology market by delivering a unique and rigorous solution for microbial fermentation applications while offering the flexibility, ease of use, and efficiency found in single-use systems.
The S.U.F. is engineered for optimal oxygen mass transfer, mixing, and temperature control, with performance comparable to conventional stainless-steel systems. S.U.F.s use fundamental engineering principles, helping to make scale-up and process development work readily streamlined. Available in 30 L and 300 L sizes, the S.U.F. consists of a fermentor tank and a standard Thermo Scientific HyPerforma S.U.F. BioProcess Container (BPC).
Benefits of single-use fermentors
Enhancements to improve performance of single-use fermentors were made to the 300 L hardware, as well as the 30 L and 300 L BPCs, to match more closely the oxygen delivery and cooling capacity performance of the stainless steel fermentors.
Hardware enhancements
The cooling jacket surface area was increased on the sides and bottom for the 300 L S.U.F. to support adequate cooling of the production process. This 35% increase jacketed surface area will enable faster cooling of the molecule production process.
Hardware and jacketing surface comparison between HyPerforma S.U.F. (300 L) and HyPerforma eS.U.F. (300 L).
BioProcessing Container (BPC) enhancements
The BPCs for 30 L and 300 L were redesigned to have three parabolic turbine impellers that increase oxygen delivery from spargers by at least four times (4x). As a result, customer and internal culture comparisons showed that oxygen supplementation was reduced 5–7 fold vs. HyPerforma S.U.F., and is comparable to performance of stainless-steel fermentors, per same strain and procedure.
Developed with standard stainless-steel fermentation design geometry (3:1 height to diameter, 5:1 turndown ratio), the S.U.F. supports scale-up and process development activities.
- 3:1 total height-to-width aspect ratio for optimal cooling surface to volume ratio
- The BPCs are highly configurable, allowing for flexibility to address specific application needs
- Complete mixing system with a water jacket for temperature control
- Drive shaft inserts into the BPC through the mixing drive motor and locks into the BPC agitator assembly of the S.U.F. BPC
- Integrally sealed ports in the S.U.F. BPC allow for addition of sensor probes and line sets
- Integrated foam sensor
- Single-use pH, DO, temperature, foam, and pressure sensors with clear optical density plug port
- Exhaust management system with options of low or high flow hydrophobic filter membranes and multiple vent filters based on gas flow needs
- High flow exhaust condenser with back-up filters, gas sparge filter line sets
- Feed, submerge fill, syringe sample, and drain line set options
Powerful agitation facilitates high oxygen transfer (kLa)and a homogeneous reaction
- 3 Rushton impellers with standard S.U.F. or enhanced parabolic with eS.U.F.
- Impellers couple directly to shaft through bearing to drive motor
- 4 baffles
- Drilled-hole sparge (DHS) with ring-like design
- Innovative exhaust management and condenser systems designed to provide reliable off-gassing and help minimize liquid loss during the fermentation process
System options
- Exhaust condenser unit (extend filter life)
- Exhaust gas vent filter heater (extend filter life, recommended 151 W for 300 L)
- Backup filter automated pinch clamp (extend filter life)
- Load cells
- Cable / tubing management tree
- Turnkey system with HyPerforma G3 process control system
- Electrical box for remote agitation or open-architecture control
S.U.F.s require a separate external temperature control unit (45 W/L min. cooling capacity recommended for high demanding cultures). You may choose an open-architecture approach or a turnkey, ready-to-use S.U.F. system.
HyPerforma Single-Use Fermentor animation
Thermo Scientific HyPerforma Single-Use Fermentor (S.U.F.) OverviewWatch this video to get a detailed technical introduction to the Thermo Scientific HyPerforma Single-Use Fermentor (S.U.F.) and learn about its unique features and advantages and how it is engineered specifically for microbial fermentation.
Cytovance and Thermo Fisher Scientific Single-Use Equipment CollaborationThis video highlights the success Cytovance has had solving complex issues with our Thermo Scientific imPULSE Single-Use Mixers (S.U.M.s) HyPerforma Single-Use Fermentors (S.U.F.s) and BioProcess Containers (BPCs).
Ordering information
CentriPAK BioProcess Containers
Next-generation centrifuge-ready BioProcess Containers provide a single-use, sterilized, closed system for cell harvesting in bioprocessing.
Resources
Bioprocessing resources
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing: