DNA Assays for HLA: SSP and RT-PCR. Back to Basics Series, Episode 1

DNA Assays for HLA: SSP and RT-PCR, Episode 1


The first episode of the 2025 Back to Basics series provides a comprehensive overview of DNA techniques used in HLA typing, focusing on Sequence-Specific Primers (SSP) and Real-Time Polymerase Chain Reaction (RT-PCR). The agenda covers the principles and practices of these techniques, highlighting their importance in the evolution of HLA typing. The presentation includes scientific fundamentals, detailed workflows, and troubleshooting tips to increase understanding and skills for running the assays. The presentation concludes by emphasizing the enduring relevance of classic DNA methods like SSP and RT-PCR in various laboratory settings, due to their effectiveness and quick turnaround time.
 

Instructor:
Linda Olsen, MPH, CHS(ACHI), MLS(ASCP)CM,
Sr. Technical Training Specialist – Molecular, Thermo Fisher Scientific

Current Time 0:00
Duration 48:30
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Stream Type LIVE
Remaining Time 48:30
 
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    HLA & Transplant Back to Basics Series ⠀⠀ ⠀

    Meet the speaker

    Linda serves as Technical Training Specialist for our molecular products, a role she has held since March 2022. She holds a bachelor’s degree in clinical laboratory science and a master’s in public health policy and management. She has worked in HLA since 2008, with experience at the University of Utah, Stanford, and ClinImmune Labs. Prior to joining the Technical Training team, she was supervisor of the HLA lab at Mayo Clinic Arizona. She has also served as an ASHI inspector and committee member. She enjoys teaching and increasing access to educational materials on the science and application of HLA.

    Linda Olsen

    These videos may contain general information relating to various medical conditions or their treatment. The information in this video is provided for informational purposes only and is not meant to be a substitute for advice provided by a doctor or other qualified health care professional. You should always consult with a doctor or other health care professional for medical advice or information about diagnosis and treatment.

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