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Basic cell culture techniques for consistent results

Gibco Cell Culture Basics provides an introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic techniques. You'll also find basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells. Start here if you are new to cell culture techniques or need to refresh your knowledge.

To accompany the tips and tricks found here, we’ve also developed a companion handbook that introduces the fundamentals of cell culture. Download a copy to keep as a frequent reference guide.

Download the handbook    Explore all topics    Watch videos

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Fetal Bovine Serum (FBS) Basics

Discover the important factors to consider in FBS selection, the market scenarios that drive FBS availability and price, as well as the important terms you must know and use when searching for the right FBS for your research needs.

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Gibco Cell Culture Basics Handbook

One Cell Biology Product Handbook

One Cell Biology Product Handbook cover

We have simplified our portfolio to show you how our most popular products and services are used as single tools or integrated system solutions. This handbook is meant to expedite your search for the right cell biology product and give you an in-depth look at how we can help accelerate research.

Learn more

artistic rendition of cow, cells and fbs bottle

Fetal Bovine Serum (FBS) Basics

Discover the important factors to consider in FBS selection, the market scenarios that drive FBS availability and price, as well as the important terms you must know and use when searching for the right FBS for your research needs.

Explore now

Gibco Cell Culture Basics Handbook

One Cell Biology Product Handbook

One Cell Biology Product Handbook cover

We have simplified our portfolio to show you how our most popular products and services are used as single tools or integrated system solutions. This handbook is meant to expedite your search for the right cell biology product and give you an in-depth look at how we can help accelerate research.

Learn more

Gibco Cell Culture Basics videos

Watch the latest Gibco Cell Culture Basics videos to learn valuable cell culture techniques to fuel your success.

Aseptic Techniques: Cell Culture Basics
The handbook and videos provide an introduction to cell culture, with a focus on aseptic technique, microbial contamination, laboratory safety and cell culture experiments.
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        1. Now Playing
          Up NextAseptic Techniques: Cell Culture Basics

          The handbook and videos provide an introduction to cell culture, with a focus on aseptic technique, microbial contamination, laboratory safety and cell culture experiments.

        2. Now Playing
          Up NextCell Culture: Cell Culture Basics

          In this video, we present the basic equipment used in cell culture and proper way to set-up a laboratory. Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment.

        3. Now Playing
          Up NextFreezing Cells: Cell Culture Basics

          This cell culture workflow video focuses on steps pertaining to freezing cells. It also contains useful information, troubleshooting guides and protocols for the experienced researcher as well.

        4. Now Playing
          Up NextPassaging Cells: Cell Culture Basics

          This video provides an introduction to cell culture, with a focus on maintaining cell health throughout the processes of culturing, freezing, thawing and passaging cells. In this video, we focus on how to passage cells.

        5. Now Playing
          Up NextThawing Cells: Cell Culture Basics

          This video provides an introduction to cell culture, with a focus on maintaining cell health throughout the processes of culturing, freezing, thawing and passaging cells. In this video, we focus on how to thaw cells.

      Gibco Cell Culture Basics topics

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      Cell Culture & Transfection Learning Center
      Access cell culture and transfection educational resources for better experiment planning and execution.

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      Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

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      Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

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