The activity and efficiency of restriction enzymes heavily depend on the buffer systems in which they are used. Each restriction enzyme requires a specific ionic environment and pH, which are provided by carefully formulated buffers. Our range of restriction enzyme buffers includes a variety of formulations designed to help optimize enzyme performance under different conditions.

Reaction buffers to support conventional restriction enzymes


Conventional enzyme 5 buffer system

The Five Buffer System helps ensure optimal reaction conditions for each restriction enzyme. The five buffers are Buffer B, Buffer G, Buffer, O, Buffer R, and Tango Buffer. We also offer a restriction enzyme buffer set.

Catalog # Name Size Price (USD) Qty
BB5 Buffer B (10X) Each
21.65

Online Exclusive

23.77
Save 2.12 (9%)
BG5 Buffer G (10X) Each
23.87
BO5 Buffer O (10X) Each
20.65

Online Exclusive

23.55
Save 2.90 (12%)
BR5 Buffer R (10X) Each
23.43
BY5 Tango Buffer (10X) Each
21.65

Online Exclusive

23.62
Save 1.97 (8%)
B30 Buffer Set for Restriction Enzymes Each
24.02


Dilution buffer

Restriction Enzyme Dilution Buffer helps ensure that restriction enzymes operating in high-salt conditions maintain their stability and activity, which is important for achieving precise and accurate cuts in complex DNA samples. This buffer's carefully balanced composition supports the enzyme's structural integrity and catalytic function, making it an excellent choice for reactions involving high-salt-dependent restriction enzymes.

Catalog # Name Size Price (USD) Qty
B19 Dilution Buffer for Restriction Enzymes Each
24.19


Optimal buffers for specific restriction enzymes

For molecular biology applications that require diluted enzymes, a specialized dilution buffer can be used. These are buffers for specific restriction enzymes.

Catalog # Name Size Price (USD) Qty
B27 AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X) Each
23.76
B57 BamHI-Lsp1109I Buffer (10X) Each
24.29
B59 BfuI Buffer (10X) Each
24.84
B31 BseXI Buffer (10X) Each
24.84
B13 Bsp143I Buffer (10X) Each
24.46
B04 Cfr10I Buffer (10X) Each
24.84
B02 Cfr9I Buffer (10X) Each
24.90
B25 Eam1105I Buffer (10X) Each
24.84
B26 Ecl136II, PacI, SacI Buffer (10X) Each
23.60
B22 Eco52I Buffer (10X) Each
24.77
B12 EcoRI Buffer (10X) Each
20.65

Online Exclusive

24.01
Save 3.36 (14%)
B29 KpnI Buffer (10X) Each
24.03
B24 SdaI Buffer (10X) Each
24.12
B23 SduI, Ppu21I Buffer (10X) Each
24.84
B28 TaqI Buffer (10X) Each
23.66

For Research Use Only. Not for use in diagnostic procedures.

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