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Shake Flasks for Suspension Culture
Shake flask features
Thermo Scientific single-use cell culture Erlenmeyer flasks are excellent for suspension culture and media preparation. Ready for mixing and storage and featuring crystal-clear polyethylene terephthalate glycol (PETG), these single-use flasks are robust and break resistant.
Standard non-vented closures help prevent spilling and contamination, and optional vented closures (0.22 µm) improves gas exchange, especially beneficial for suspension cell culture.
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Our angled flask (Cat. No. 4115-5000) has also shown to be the only flask tested capable generating larger protein titers in CHO cells due to its proprietary design (see Figure 1).
Figure 1. Protein expression levels in 5 L shake flasks from different suppliers. Protein titers were determined for ExpiCHO cells transfected in a 1.5 L starting volume using the Max Titer (32°C) protocol with a three-feed strategy in 5 L shake flasks (N = 2).
Don’t take our word for it. See our application notes regarding how to help increase your potential for each Expi kit.
Product features include:
- Molded-in graduation for accurate volume assessment
- Non-pyrogenic, non-cytotoxic
- Optional vented caps for gas exchange
- Gamma irradiation sterilization (per ISO 1137-2)
- DNase-, RNase-, and human DNA-free
- BSE and TSE risk-free
Shake flask volume information
| Capacity | Outer diameter | Case size |
|---|---|---|
| 125 mL | 66.3 mm | 24 |
| 250 mL | 82.6 mm | 12 |
| 500 mL | 101.6 mm | 12 |
| 1,000 mL | 129.8 mm | 6 |
| 2,000 mL | 161.5 mm | 4 |
| 2,800 mL | 203.2 mm | 4 |
| 5,000 mL | 214 mm | 4 |
Order sterile single-use shake flasks
Separated below by cap type, sterile single-use shake flasks are available with plain and baffled bottoms. These sterile single-use Erlenmeyer flasks for cell culture are:
- Baffled-bottom flasks are designed to provide additional aeration and gas exchange with low shear to help maintain cell viability
- Sterile and single use to help reduce contamination risk
- BPA-free
- Collapsible when autoclaved, helps reduce biohazardous waste
- Graduated to enable rapid volume assessment
Resources
Application notes
Brochures
Stylesheet for Classic Wide Template adjustments
For Research Use Only. Not for use in diagnostic procedures.