Technical Resources for Qubit Fluorometers

Online and downloadable materials for Qubit Fluorometric Quantitation

The manuals, brochures, application notes, downloads, demos, videos, and other resources in this section will help you get the most out of your Qubit Fluorometer.

 

Documentation and literature

Demos and videos

Qubit 4 Interactive 3D virtual tour

Tour the features and capabilities of the Qubit 4 Fluorometer with our hands-on interactive demo.

Qubit 4 fluorometer virtual tour

Qubit Flex Interactive 3D virtual tour

Tour the features and capabilities of the Qubit Flex Fluorometer with our hands-on interactive demo.

Qubit Flex fluorometer virtual tour

Qubit Flex SmartStart interactive demo

Every Qubit Flex Fluorometer includes access to a self-paced online tutorial and reference experience designed to help you get up and running quickly.

Interactive screen of the digitized demonstration of the Qubit Flex instrument
1:45

Quantify DNA, RNA, or protein with the Qubit 4 Fluorometer

Quickly quantitate the amount of DNA, RNA, or protein for proper preparation of the next step in your experiment. The quantitation process is simple, fast and even includes a built-in Reagent Calculator.
2:48

Qubit Flex Fluorometer demonstration video

Learn how to increase your nucleic acid quantification throughput with the Qubit Flex Fluorometer. Simply measure up to 8 samples at one time with convenient reagent, molarity, assay range, and normalization calculators.
4:36

Quantitation: Comparing the Qubit fluorometer to the Nanodrop One UV/Vis absorbance

See how both fluorescence and UV-Vis technologies can be used for DNA, RNA or protein quantification for all your downstream experiments.
2:35

Invitrogen Qubit 4 Fluorometer: Quantitation

Learn how to quantitate a variety of samples using the Qubit 4 Fluorometer, including ssDNA, dsDNA, RNA, microRNA and protein in just 2 simple steps with accuracy across a relevant dynamic range for most applications.
2:05

Invitrogen Qubit 4 Fluorometer: Managing Data

Learn how to manage your data generated using the Invitrogen Qubit 4 fluorometer, including storing up to 1000 results, transferring results to a computer, editing and saving the units of measure for results.
0:00

Invitrogen Qubit 4 Fluorometer: Additional Features

Learn about the many additional features for the Invitrogen Qubit 4 Fluorometer, including how to access data, run a verification test, change languages, create custom assays, add new assays from thermofisher.com/myqubit, and edit the display.
0:00

Invitrogen Qubit 4 and RNA Quantitation and Qualification

Describes how to use the Qubit 4 Fluorometer and Qubit RNA IQ assay to measure RNA integrity and quality in preparation for use in RNASeq and other applications where viable RNA is required for success.

Register your instrument

Register your instrument to receive notifications of firmware updates.

Firmware updates

Download the latest firmware for Qubit 4 and Qubit Flex Fluorometers here.

Qubit Flex SAE Software Solution for 21 CFR Part 11 Compliance Support

Service Support

Qubit Flex Fluorometers offer installation qualification (IQ) and operational qualification (OQ) services performed by our manufacturer-trained and -certified field service engineers (FSEs). This service will ensure that the Qubit Flex is properly installed and functioning according to manufacturer’s specifications. 

 

In addition to the instrument IQ/OQ, an additional service to perform IQ/OQ for the Qubit Flex SAE Software can be completed during the same visit. The SAE IQ/OQ will ensure that the SAE Software is properly installed and that the connection to the instrument can be made. Further, it will include testing that verifies proper functionality of the SAE Software. The SAE IQ/OQ can only be completed in conjunction with an instrument IQ/OQ.

Frequently asked questions (FAQs)

Here are frequently asked questions and answers about Qubit Fluorometers and Qubit fluorometric quantitation. We invite you to contact Technical Support  if you have additional questions or need more information.

Comparison to UV absorbance

General technical questions

Quantifying protein and nucleic acids

Device questions

How to create custom MyQubit assays (Qubit 3.0 and 4)

MyQubit functionality allows you to create new assays for the Qubit 3.0 and 4 Fluorometers in minutes, using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. Your new assays can be optimized versions of existing fluorescence-based assays or can be completely novel.

 

To create custom MyQubit assays for the Qubit 2.0 Fluorometer, see Resources for previous Qubit models.

Step 1: Collect data and define constants for MyQubit assays

Collecting the data. Prepare samples using your assay reagents and read them in Fluorometer mode on the Qubit instrument. Fluorometer mode allows you to manually select the excitation light source of your choice, blue LED or red LED, while reading raw fluorescence values in both the green and far red emission channels. The values can be used to generate data to develop your new assay.

Defining the constants. We recommend that all users new to developing MyQubit assays use the MyQubit EZ Guide Excel spreadsheet on your computer to process your Fluorometer mode data and generate the parameters for your assays. The Guide allows you to enter Fluorometer mode readings collected from samples of known concentrations and then calculates constants that can be used to define the shape of the standard curve that will be used to calibrate the new assay.

 

Simply enter the fluorescence values obtained in Fluorometer mode on the Qubit Fluorometer, along with the corresponding concentrations of the analyte. Once replicate data has been entered into the spreadsheet, adjust the N and K parameters to optimize the curve fit algorithm. Your goal is to adjust N and K to achieve the lowest possible Quality score as shown in the example.

MyQubit EZ Guide

Step 2: Define your assay with the MyQubit Assay Design Tool

You can use the MyQubit Assay Design Tool to create the MyQubit .qbt file that defines your new assay. You can then upload the .qbt file to run on your Qubit 3.0 or 4 Fluorometer.

 

In the MyQubit assay design tool, you enter the parameter values for your custom assay as calculated on the MyQubit EZ Guide spreadsheet. Fields may appear or disappear as you select values. Hover the cursor over the help icons for guidance on filling out the fields in the tool.

My Qubit Assay Design Tool

Step 3: Upload your assay to your Qubit 3.0 or 4 Fluorometer

To upload your new assay .qbt file, you can import it to your instrument from a USB drive or directly from an external computer via a USB cable. Your Qubit Fluorometer will guide you through the process of uploading new assays. New assays can be permanently uploaded without affecting existing assays.

Import assays from a USB drive

1.     Transfer the MyQubit assay (.qbt) file(s) you wish to install to the root (top-level) directory of a USB drive.

Do not download the .qbt file(s) to a folder in the USB drive.

2.     Insert the USB drive containing the new assay file(s) into the USB port of the Qubit Fluorometer.

3.     Go to the Settings screen and touch Import new assay. The instrument searches the USB drive for assay file(s) and displays their names on the Import new assay screen.

Settings and Import new assay screens

4.     Touch to select the assay(s) you wish to import. (Touch an assay again to deselect it.) You can select one or more assays to import.

5.     Touch Next. The Import new assay screen displays the name of the first assay being imported and lists the destination folders where you can save them. Each folder can hold up to 9 assays.

6.     Select the destination folder for the new assay and touch Save. You’ll see your choice on the Verification screen (step 8).

Alternatively, touch New folder to create a new destination folder (step 7).

7.     On the New folder screen, touch the New folder text field. On the keyboard that pops up, type a folder name of up to 11 characters. The name can include spaces except at the beginning or end.

Touch Enter, and then touch Done to return to the Import new assay screen. The new folder will appear in the folder list.

Select the new destination folder and touch Save. You’ll see your choice on the Verification screen.

8.     On the Verification screen, touch Done to complete the import. If you’re importing only one assay, you’ll return to the home screen.

If you’re importing multiple assays, you’ll return to the Import new assay screen where you can select the destination folder for the next assay to be imported.

Saving imported new assay

Import assays from a computer

You can transfer MyQubit assay (.qbt) files directly from your computer to your Qubit 3.0 or 4 Fluorometer using a USB cable.

Note: The USB cable must remain connected to both the Qubit Fluorometer and the computer throughout this procedure.

  1. If necessary, download the MyQubit assay (.qbt) file to your computer.
  2. Connect the Qubit Fluorometer to your computer using a USB cable. Your computer should recognize the fluorometer as an external device.
  3. Copy and paste (or click and drag) the .qbt file(s) you wish to import to the Qubit instrument window on your computer.
    •  Note: The assays will not be visible on the Qubit Fluorometer until you perform the next step.
  4. Go to the Settings screen on the Qubit Fluorometer and touch Import new assay. On the Import new assays screen, you’ll see the names of assays found on the computer.
  5. Follow steps 4­–8 of the import procedure described in Import assays from a USB drive above.

Step 4: Run your assay

Once uploaded, your new MyQubit assay is ready to use. Refer to the Qubit 4 product manual for a detailed description of instrument function and assay workflow.

Connecting to a Windows® computer (Qubit 3.0 or 4)

Connecting a Qubit 3.0 or 4 Fluorometer to a computer running the Windows® operating system requires installation of a driver. Download both the driver and installation instructions below.

Citations

2022

  • Khan SA, Gudeta DD, Chon J, et al. Whole-genome sequences of nine hospital-associated methicillin-susceptible Staphylococcus aureus strains." Microbiol Resour Announc. 2022; 11: e0118521. PubMed
  • Lopez D, Roberts J, Bourgeois M, et al. Infection clusters can elevate risk of diagnostic target failure for detection of SARS-CoV-2. PLoS one 2022; 17: e0264008. PubMed

2021

  • Kaspar JR. Complete genome sequence of Streptococcus oralis 34." Microbiol Resour Announc. 2021; 10: e00760–21. PubMed
  • Ng DL, Granados AC, Santos YA, et al. A diagnostic host response biosignature for COVID-19 from RNA profiling of nasal swabs and blood. Sci adv. 2021; 7: eabe5984. PubMed
  • Redila CD, Prakash V, Nouri S. Metagenomics analysis of the wheat virome identifies novel plant and fungal-associated viral sequences. Viruses. 2021; 13, 2457. PubMed

2020

  • Li J, Zhao S, Lee M, et al. Reliable tumor detection by whole-genome methylation sequencing of cell-free DNA in cerebrospinal fluid of pediatric medulloblastoma. Sci Adv. 2020; 6: eabb5427. PubMed
  • Lu R, Zhao X, Li J. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020; 395: 565–574. PubMed
  • Mondelo-Macía P, Rodríguez-López C, Valiña L, et al. Detection of MET alterations using cell free DNA and circulating tumor cells from cancer patients. Cells. 2020; 9: 522. PubMed
  • Wright K, de Silva K, Purdie AC, Plain KM. Comparison of methods for miRNA isolation and quantification from ovine plasma." Sci Rep. 2020; 10: 825. PubMed

2019

  • Hosein AN, Huang H, Wang Z, et al. Cellular heterogeneity during mouse pancreatic ductal adenocarcinoma progression at single-cell resolution." JCI Insight. 2019; 5: e129212. PubMed
  • Krasnenko AY, Stetsenko IF, Klimchuk OI, et al. Ultra-low coverage sequencing as the most accurate library quantification. SpringerLink

2018

  • Zingg D, Debbache J, Peña-Hernández R, et al. EZH2-mediated primary cilium deconstruction drives metastatic melanoma formation." Cancer cell. 2018; 9: 69–84. PubMed
  • Ponti G, Maccaferri M, Manfredini M, et al. The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients. Clin Chim Acta. 2018; 479: 14–19. PubMed

Additional references

  • Garcia-Elias A, Alloza L, Puigdecanet E, et al. Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs. Sci Rep. 2017; 7: 7725. PubMed
  • Kumar D, Panigrahi MK, Suryavanshi M, Mehta A, Saikia KK. Quantification of DNA extracted from formalin fixed paraffin-embeded tissue comparison of three techniques: Effect on PCR efficiency." J Clin Diag Res. 2016; 10: BC01­–BC03. PubMed
  • Mardis E, McCombie WR. Library quantification: fluorometric quantitation of double-stranded or single-stranded DNA samples using the qubit system. Cold Spring Harb Protoc. 2017; 2017: pdb-prot094730. PubMed
  • Simbolo M, Gottardi M, Corbo V, et al. DNA qualification workflow for next generation sequencing of histopathological samples. PLoS ONE. 2013; 8: e62692. PLOS

Educational resources

Educational resources for Qubit Fluorometric Quantitation include online materials on nucleic acid and protein quantification and analysis.

Nucleic acid quantification information and support

Check out these free resources for background and support materials about Qubit Fluorometric DNA and RNA quantification.

Image of double stranded DNA disassociated

Protein Biology Learning Center

Our free introduction to protein analysis methods and technologies includes articles, videos, guided learning, and more resources on proteins and ways to study them. The Overview of protein assays methods might be a good place to start.

Protein illustration

Resources for previous Qubit models

Find manuals, brochures, application notes, downloads, demos, videos, and other resources to help you get the most out of your older Qubit Fluorometer.

For Research Use Only. Not for use in diagnostic procedures.