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Transfection Information
This resource is designed to provide researchers with comprehensive knowledge and practical guidance on transfection techniques, which are essential for introducing nucleic acids into eukaryotic cells. Whether you are new to transfection or looking to optimize your current protocols, find information, including detailed protocols, troubleshooting tips, and recommendations on selecting the right transfection reagents and methods for your specific applications. Read up on the latest advancements and best practices in transfection to enhance your research outcomes and achieve effective and reliable gene delivery in your cell culture experiments.
Finding that optimal match
Transfection is a technique used to introduce foreign nucleic acids (DNA or RNA) into eukaryotic cells to study gene function, protein expression, or to create genetically modified cells. It works by using various methods such as chemical reagents, electroporation, or viral vectors to facilitate the uptake of nucleic acids across the cell membrane. Transfection differs from transformation, which refers to the genetic alteration of prokaryotic cells (like bacteria) through the uptake of foreign DNA from the surrounding environment. The key difference between transfection and transduction is the method of delivery; transfection uses non-viral methods to introduce nucleic acids into cells, whereas transduction involves the use of viral vectors to transfer genetic material into cells. Both techniques are important tools in molecular biology but are applied to different types of cells and processes.
Whether for protein expression, gene expression modulation, or genome editing, efficient, effective transfection relies on an optimal match between the cultured cells, the transfection reagent or equipment, and post-transfection care and maintenance. Get the information you need to get the results you're aiming for.
Transfection features
Handbook
Download this free guide that outlines a collection of superior transfection and TALs/CRISPR genome engineering products designed to work together to help answer important scientific questions faster and with less effort.
Application note
This application note provides an overview for the use of Lipofectamine MessengerMAX reagent is used for high-efficiency transfection of neuronal cell models, enabling improved results and simplified workflows for applications such as genome editing. We also compare Lipofectamine MessengerMAX reagent to other gene delivery solutions and highlight the advantages and drawbacks of each approach.
Transfection protocols—by product
We've collected protocols for all of our transfection reagents (21 in total!) in one spot. Once you've located the reagent that matches your requirements from the list, the protocol is just a click away.
Transfection protocols—by cell type
After you've located your cell line from the extensive list, you'll be able to see the recommended transfection reagent and a link to the protocol.
Cell Culture & Transfection Resource Library
Browse cell culture & transfection learning center content by collection (resource type)—application notes, case studies, videos, webinars, and white papers.