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Anti-Clumping Agent
GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densitiesRead more
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| Catalog Number | Quantity |
|---|---|
| 0010057AE | 20 mL |
| 0010057DG | 100 mL |
Catalog number 0010057AE
Price (USD)
88.00
Each
-
Quantity:
20 mL
Price (USD)
88.00
Each
GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.
• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin-free and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and delivery
Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping” state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.
Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.
For actual cell dissociation, we recommend our animal origin-free TrypLE™ products.
Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.
Animal Origin-free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.
Convenient and Customizable Packaging
Previously, Anti-Clumping Agent was only available in a 20 mL bottle. However, it is now available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking on the 'Customize this Product' link under the 'Add to Cart' button above or by emailing us at CustomMedia@lifetech.com.
Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Services group.
For Research Use Only. Not for use in diagnostics procedures.
• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin-free and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and delivery
Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping” state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.
Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.
For actual cell dissociation, we recommend our animal origin-free TrypLE™ products.
Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.
Animal Origin-free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.
Convenient and Customizable Packaging
Previously, Anti-Clumping Agent was only available in a 20 mL bottle. However, it is now available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking on the 'Customize this Product' link under the 'Add to Cart' button above or by emailing us at CustomMedia@lifetech.com.
Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Services group.
For Research Use Only. Not for use in diagnostics procedures.
Specifications
Cell LineCHO, Insect Cells, 293 (HEK)
Cell TypeMammalian Cells
Culture TypeSuspension Cell Culture
FormatBottle(s)
Product LineGibco™
Quantity20 mL
Shipping ConditionRoom Temperature
SourceAnimal origin-free
ClassificationAnimal Origin-free, Chemically-defined, Protein-free, Serum-free
FormLiquid
Product TypeAnti-clumping Reagent
pH6 to 8
Unit SizeEach
Contents & Storage
Store at 2 to 8°C
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Figures
Example of Minimized Clumping Due to Anti-Clumping Agent
Percent Increase Viable Cell Density from Anti-Clumping Agent
Example of Cell Clumping
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Lot #Certificate TypeDateCatalog Number(s)
2562584Certificate of AnalysisFeb 10, 20230010057AE, 0010057DG
2487545Certificate of AnalysisFeb 10, 20230010057AE, 0010057DG
2487543Certificate of AnalysisFeb 10, 20230010057AE, 0010057DG
2487541Certificate of AnalysisFeb 10, 20230010057AE, 0010057DG
2487539Certificate of AnalysisFeb 10, 20230010057AE, 0010057DG
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Safety Data Sheets
SDSLimited Use Label Licenses (LULL)
'
Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'Frequently asked questions (FAQs)
Use anti-clumping agent at a dilution of 1:250 to 1:1000 in culture medium. Test for impact on the cell culture and reduce concentration as the cell clumping is reduced.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
When cells meant to be grown in suspension are grown in static culture, they may form clumps. These clumps will severely limit transfection efficiency and protein expression. It is suggested that FreeStyle 293 cells in FreeStyle media and CHO-S cells in CD-CHO or CHO-SFM are grown in agitated suspension to reduce the appearance of clumps. However, if clumps do form, you can try the following protocol to select for cells that don't form clumps:
- Transfer cells into an appropriate size centrifuge tube that will hold the entire cell suspension.
- Allow cells to sit undisturbed for about 5 minutes. The time can vary depending on the specific cell line. Larger cell clumps will settle to the bottom of the tube.
- Collect cells from the upper portion of the tube to passage into a new flask.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
There are a number of factors that can contribute to this:<
1) Presence of calcium and magnesium ions. Wash cells in a balanced salt solution without calcium and magnesium. Gently pipette cells to obtain a single cell suspension.
2) Mycoplasma contamination. Segregate culture and test for mycoplasma infection. Clean hood and incubator. If culture is contaminated, discard.
3) Cell lysis and release of DNA resulting from overdigestion with proteolytic enzymes. Treat cells with DNase I.
4) Certain suspension cell type try to aggregate when they are not under optimal agitation speed and optimization is required.
5) Certain suspension cell type try to aggregate in bioreactors and it is recommended to use Anti-Clumping Agent (Cat. No. 0010057AE).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.