Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose™ 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose™ 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.19% Kathon CG/ICP).
Applications: Immunoglobulin affinity purification and Immunoprecipitation.
Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:
• all four subclasses of human IgG • mouse IgG • rabbit IgG • guinea pig IgG • rat IgG • goat IgG • sheep IgG • cow IgG • horse IgG
Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly to IgG from dog.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Type
Protein G Sepharose
Product Line
Sepharose™
Column Type
Affinity
Stationary Phase
Protein G
Quantity
25 mL
Form
Suspension
Contents & Storage
This product is supplied as a 50% suspension (25 ml Recombinant Protein G-Sepharose™ 4B + 25 ml PBS) Wet Ice