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SuperScript™ VILO™ cDNA Synthesis Kit
Upgrade to SuperScript IV VILO Master Mix for superior performance
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SuperScript™ VILO™ cDNA Synthesis Kit
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit isRead more
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Catalog NumberNo. of Reactions
1175405050 Reactions
11754250250 Reactions
Catalog number 11754050
Price (USD)
694.00
Special offer
Online exclusive
Ends: 21-Jul-2025
786.00
Save 92.00 (12%)
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No. of Reactions:
50 Reactions
Request bulk or custom format
Price (USD)
694.00
Special offer
Online exclusive
Ends: 21-Jul-2025
786.00
Save 92.00 (12%)
Each
Add to cart

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatKit
No. of Reactions50 Reactions
Optimal Reaction Temperature42°C
Quantity50 reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Size (Final Product)Up to 10 kb
Starting MaterialRNA
TechniqueReverse Transcription
Detection MethodqPCR
For Use With (Application)Real Time PCR (qPCR)
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
Unit SizeEach
Contents & Storage

• 5X VILO Reaction Mix, 200 μL
• 10X SuperScript III Enzyme Blend, 100 μL

Store at –5 to –30°C.

Frequently asked questions (FAQs)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?

The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript

The SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

In what concentration range of total RNA does the SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) work?

  • Lower limit of RNA concentration: 1.0 pg
  • Upper limit of RNA concentration: 2.5 µg


    • Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center

    What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

    Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).

    View all SuperScript™ VILO™ cDNA Synthesis Kit FAQs

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