Real-time PCR master mixes for gene expression analysis

Applied Biosystems qPCR and RT-qPCR master mixes are engineered to deliver exceptional specificity and reliability for your gene expression analysis. Broadly compatible and supported by trusted expertise, our master mixes are ideal for applications in infectious disease research, cancer research, biomarker discovery, and pharmacogenomics. Whether you need single-target SYBR green dye-based detection or advanced TaqMan probe-based multiplexing, our reagents enable fast, accurate, and reproducible results across a wide range of target concentrations. Accelerate your path to discovery with the accuracy and consistency you can trust.

Compare SYBR Green vs TaqMan chemistries for gene expression analysis

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PCR and RT-qPCR master mixes for gene expression

For optimal performance, we recommended using one of the following qPCR master mixes for gene expression analysis.

 

 

 


TaqMan Fast Advanced Master Mix


TaqMan Fast Virus 1-Step master mixes

 PowerTrack SYBR Green Master Mix*
Overview Bench-top stable reagent with robust performance in singleplex or duplex reactions Highly sensitive, inhibitor-tolerant reagents capable of 4-target multiplex Universal 2X reagent master mix  with built-in visual indicators for accurate reaction setup

Chemistry

Probes, 2-step qPCR

Probes, 1-step RT-qPCR

SYBR Green dye, 2-step qPCR
Instrument compatibility Any qPCR instrument, from any manufacturer

Target

cDNA

RNA

cDNA

Concentration

2X

4X

2X

Multiplexing and passive reference dye

With ROX:  up to 2 targets

Available in 2 versions:
With ROX: up to 3 targets

No ROX: 3 or more targets 

With ROX: 1 target

Cycling mode: run time

Fast:  ~40 min

Standard: ~50 min

Fast: ~60 min

Standard: ~90 min

Fast: ~30 min

Standard: ~60 min

Carryover contamination control

2 versions:

With UNG

No UNG

No UNG

With UNG
Sample tracking dye No No Yes

Reverse transcriptase

SuperScript IV VILO Master Mix

Thermostable MMLV RT included in master mix

SuperScript IV VILO Master Mix

Assay recommendations

TaqMan Gene Expression assays

TaqMan Gene Expression assays

qPCR primers

Product page

Check price or order now

Check price or order now

Check price or order now

* For SYBR Green detection: when you need up to 72 hours of benchtop stability, choose PowerUp SYBR Green Master Mix in combination with High-Capacity RNA-to-cDNA Kit Reverse Transcriptase.

Reagents for diagnostic testing, bioanalysis, and clinical trial assay development

For biomarkers or drug targets moving beyond research and development, we offer master mixes that scale with growing study size and complexity. TaqPath general purpose reagents are made in ISO 13485-certified and FDA-registered facilities, following cGMP principles to enable lot-to-lot reproducibility and consistent Ct values. Maintain confidence in the precision of your gene expression data, while driving efficiency with master mixes that are optimized for high-throughput workflows and multiplex analysis

TaqPath qPCR general purpose reagents

Other real-time PCR master mixes

Our master mixes have long supported researchers with their qPCR gene expression analysis. However, for optimal results we recommend upgrading to one of our newer master mixes listed in the table above, which offer superior performance.

 

Legacy TaqMan Master Mixes include:

Legacy SYBR Green master mixes include:

Complementary products for gene expression analysis

Elevate qPCR performance and eliminate uncertainty with our high-performance products for every step of your workflow. Designed to work seamlessly with our master mixes, our sample preparation kits, reverse transcription reagents, and TaqMan Assays ensure high-quality results every time. Achieve reliable qPCR results with our comprehensive solutions

TaqMan Predesigned Gene Expression Assays

Choose from over 2.8 million predesigned assays covering more than 30 species to detect virtually any gene transcript with reliable precision.  

SuperScript VILO Master Mix for two-step RT-qPCR

SuperScript VILO Master Mix is a first-strand cDNA synthesis mix featuring Superscript IV Reverse Transcriptase for reliable synthesis and high cDNA yields.

Specificity in gene expression analysis

Target specificity is a critical and sometimes challenging aspect of gene expression analysis. Without it, your data can be compromised, leading to inaccurate results and potentially flawed conclusions. Before running your next experiment, read our white paper and infographic to learn why specificity can be challenging in gene expression analysis. Review the role of qPCR detection chemistry in assay specificity and discover key insights to help you achieve reliably accurate results.

Educational Resources for Gene Expression Analysis

For Research Use Only. Not for use in diagnostic procedures.

*For Laboratory Use.

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1x1 image pixel for data collection