Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
카탈로그 번호 | 수량 |
---|---|
15338100 | 1 mL |
A12621 | 0.1 mL |
15338030 | 0.3 mL |
Yes, all of our lipid transfection reagents are stable at room temperature for months.
Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.
We recommend Lipofectamine 3000 for delivery of vector-based RNAi but for direct transfection of synthetic siRNA, we recommend using Lipofectamine RNAiMAX. For in vivo siRNA experiments, we recommend Invivofectamine 3.0 Reagent.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
We recommend using Lipofectamine 3000 which demonstrates much higher transfection efficiency and protein expression over all other transfection reagents for suspension, hard-to-transfect, and primary cells. Review Lipofectamine 3000 data for over 60 cell lines here https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-3000.html. For highest transfection efficiencies, we recommend the Neon Transfection System for Jurkat (94%) and K562 (90%) cells.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
We have tested four primary cell lines: HuVEC (human ubilical vein endothelial cells), NHFF (normal human foreskin fibroblast), MJ90, and NDHF with transfection efficiencies between 5-25%. Instead, we recommend using Lipofectamine 3000 which shows highest transfection efficiency for all cell types and optimize by varying the DNA and lipid ratio. Alternatively, the Neon Transfection System shows 75-80% transfection efficiency. Please visit our Neon cell specific protocol database for more information: https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection---selection-misc/neon-transfection-system/neon-protocols-cell-line-data.html?icid=fr-neon-3
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Lipofectamine LTX was designed for delivery of plasmid DNA with minimal cytotoxicity. We do not recommend using it for siRNA delivery. We recommend using Lipofectamine 3000 with the P3000 Enhancer for co-transfection applications.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
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