Lipofectamine™ LTX Reagent with PLUS™ Reagent
Lipofectamine™ LTX Reagent with PLUS™ Reagent
Invitrogen™

Lipofectamine™ LTX Reagent with PLUS™ Reagent

Lipofectamine™ LTX Reagent은 transfection이 어려운 세포의 유전자 발현 연구에 새로운 첨단 해결책을 제공합니다. 어렵고 민감한 일차 세포주의 transfection에는 Lipofectamine™ LTX자세히 알아보기
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카탈로그 번호수량
153381001 mL
A126210.1 mL
153380300.3 mL
카탈로그 번호 15338100
제품 가격(KRW)
995,000
온라인 행사
Ends: 30-Jun-2025
1,105,000
할인액 110,000 (10%)
Each
-
카트에 추가하기
수량:
1 mL
제품 가격(KRW)
995,000
온라인 행사
Ends: 30-Jun-2025
1,105,000
할인액 110,000 (10%)
Each
카트에 추가하기
Ask our AI about this Product
Lipofectamine™ LTX Reagent은 transfection이 어려운 세포의 유전자 발현 연구에 새로운 첨단 해결책을 제공합니다. 어렵고 민감한 일차 세포주의 transfection에는 Lipofectamine™ LTX Reagent의 효율성(efficiency)과 편이성(convenience), 관용성(gentleness)을 따라올 만한 플라스미드 DNA 특이 transfection reagent가 없습니다. Lipofectamine™ LTX Reagent는 100% 동물유래성분이 없는 성분들로부터 합성된 제품으로 연구실험이나 세포주에서 BSE나 바이러스같은 동물원성 질병의 유무를 쉽게 검사할 수 있습니다. 원산지 증명서(C/O)는 기술 지원부에 연락해 받을 수 있습니다. Lipofectamine™ LTX Reagent의 특징:
• 우수한 전달력 - 어려운 일차 세포를 포함해 다양한 세포 유형의 transfection에 높은 효율성 보장
• 우수한 성능 - 높은 transfection 효율과 최대(>90%) 세포 생활성 제공
• 최적화 필요성 절감 - 대부분의 세포주에 신속하고 단순한 protocol 제공

일차 세포 transfection 효율 향상

일차 세포는 transfection이 어려운 경우가 많았습니다. Lipofectamine™ LTX Reagent과 PLUS™ Reagent을 함께 사용해 일차 신경전구세포 등의 일차 세포 transfection 문제를 해결합니다(그림 1).

transfection이 어려운 세포에 우수한 전달

RAW267.3 포식세포에서 높은 수준의 단백질 발현을 달성해야 하는 경우 Lipofectamine™ LTX Reagent이 경쟁사 transfection 시약보다 우수한 성능을 보입니다(그림 2). 이전에 transfection이 어려웠던 세포에서 high-level transgene expression을 달성하여 이전에 구하지 못했던 결과를 얻을 수 있습니다.

폭넓은 세포주 호환성

어려운 일차 세포 transfection 성능 외에도 Lipofectamine™ LTX Reagent는 다른 transfection 시약에 민감한 세포주를 포함해 확립된 세포주 transfection에 다용도로 사용할 수 있습니다. 이런 세포 유형에 권장되는 transfection 조건은 www.invitrogen.com/transfection에서 찾아볼 수 있습니다.

간편하고 신속한 혈청 호환 protocol

Lipofectamine™ LTX Reagent은 transfection 화학물 제거나 transfection 후 배지 변경/추가가 필요하지 않은 간단한 protocol을 제공합니다. 직접 작업하는 시간을 줄이고 빨리 결과를 얻으세요.

이 제품은 진단용으로만 사용가능합니다. 동물 또는 사람의 치료 또는 진단용으로 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
분류Animal Origin-Free
용도(애플리케이션)Transfection
고처리량 호환성High-throughput Compatible
제품라인Lipofectamine™
제품 유형Transfection Reagent
수량1 mL
혈청 호환 가능Yes
배송 조건Wet Ice
셀 유형Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Plasmid DNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Each unit contains one vial of Lipofectamine™ LTX Reagent (1 mL) and one vial of Plus Reagent (0.85 ml). Store at 2–8°C.
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그림

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문서 및 다운로드

Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
3182050Certificate of Analysis2025년 6월 03일A12621
3109684Certificate of Analysis2025년 6월 02일15338100
3098515Certificate of Analysis2025년 5월 05일15338030
3078379Certificate of Analysis2025년 3월 21일A12621
3078359Certificate of Analysis2025년 3월 12일15338100
시험 성적서 관련 5 개의 결과가 검색되었습니다.

Safety Data Sheets | 물질 안전 보건 자료

프로토콜

자주 묻는 질문(FAQ)

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

We recommend Lipofectamine 3000 for delivery of vector-based RNAi but for direct transfection of synthetic siRNA, we recommend using Lipofectamine RNAiMAX. For in vivo siRNA experiments, we recommend Invivofectamine 3.0 Reagent.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

We recommend using Lipofectamine 3000 which demonstrates much higher transfection efficiency and protein expression over all other transfection reagents for suspension, hard-to-transfect, and primary cells. Review Lipofectamine 3000 data for over 60 cell lines here https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-3000.html. For highest transfection efficiencies, we recommend the Neon Transfection System for Jurkat (94%) and K562 (90%) cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

We have tested four primary cell lines: HuVEC (human ubilical vein endothelial cells), NHFF (normal human foreskin fibroblast), MJ90, and NDHF with transfection efficiencies between 5-25%. Instead, we recommend using Lipofectamine 3000 which shows highest transfection efficiency for all cell types and optimize by varying the DNA and lipid ratio. Alternatively, the Neon Transfection System shows 75-80% transfection efficiency. Please visit our Neon cell specific protocol database for more information: https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection---selection-misc/neon-transfection-system/neon-protocols-cell-line-data.html?icid=fr-neon-3

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Lipofectamine LTX was designed for delivery of plasmid DNA with minimal cytotoxicity. We do not recommend using it for siRNA delivery. We recommend using Lipofectamine 3000 with the P3000 Enhancer for co-transfection applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

인용 및 참조 문헌 (32)

인용 및 참조 문헌
Abstract
Interaction between sterol regulatory element-binding proteins and liver receptor homolog-1 reciprocally suppresses their transcriptional activities.
Authors:Kanayama T,Arito M,So K,Hachimura S,Inoue J,Sato R
Journal:The Journal of biological chemistry
PubMed ID:17283069
Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes.
Authors:Wakamatsu K,Ogita H,Okabe N,Irie K,Tanaka-Okamoto M,Ishizaki H,Ishida-Yamamoto A,Iizuka H,Miyoshi J,Takai Y
Journal:The Journal of biological chemistry
PubMed ID:17472964
Cytohesins are cytoplasmic ErbB receptor activators.
Authors:Bill A, Schmitz A, Albertoni B, Song JN, Heukamp LC, Walrafen D, Thorwirth F, Verveer PJ, Zimmer S, Meffert L, Schreiber A, Chatterjee S, Thomas RK, Ullrich RT, Lang T, Famulok M,
Journal:Cell
PubMed ID:20946980
'Signaling by ErbB receptors requires the activation of their cytoplasmic kinase domains, which is initiated by ligand binding to the receptor ectodomains. Cytoplasmic factors contributing to the activation are unknown. Here we identify members of the cytohesin protein family as such factors. Cytohesin inhibition decreased ErbB receptor autophosphorylation and signaling, ... More
Cytoskeletal anchoring of GLAST determines susceptibility to brain damage: an identified role for GFAP.
Authors:Sullivan SM, Lee A, Björkman ST, Miller SM, Sullivan RK, Poronnik P, Colditz PB, Pow DV,
Journal:J Biol Chem
PubMed ID:17684014
'Glial fibrillary acidic protein (GFAP) is an enigmatic protein; it currently has no unambiguously defined role. It is expressed in the cytoskeleton of astrocytes in the mammalian brain. We have used co-immunoprecipitation to identify in vivo binding partners for GFAP in the rat and pig brain. We demonstrate interactions between ... More
Oxidative stress modulates complement factor H expression in retinal pigmented epithelial cells by acetylation of FOXO3.
Authors:Wu Z, Lauer TW, Sick A, Hackett SF, Campochiaro PA,
Journal:J Biol Chem
PubMed ID:17558024
'Age-related macular degeneration (AMD), the leading cause of severe vision loss in the elderly, is a complex disease that results from genetic modifications that increase susceptibility to environmental exposures. Smoking, a major source of oxidative stress, increases the incidence and severity of AMD, and antioxidants slow progression, suggesting that oxidative ... More
32 total citations

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