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IPTG
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IPTG
Invitrogen™

IPTG

IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable forRead more
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Catalog NumberQuantity
155290191 g
Catalog number 15529019
Price (USD)
92.65
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98.00
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Quantity:
1 g
Price (USD)
92.65
Online Exclusive
98.00
Save 5.35 (5%)
Each
Add to cart

IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures. IPTG binds to the lac repressor, releasing it from the lac operator, and thereby allowing the transcription of genes in the lac operon, such as the gene coding for β-galactosidase.

In addition, IPTG is one of the most efficient molecular inducers for promoter’s transcriptional activity and is used extensively for heterologous protein expression induction in E. coli. Though mainly used in cell culture assays, publications demonstrated the use IPTG in vivo in animal models too.

Primary applications
• Blue-white screening—induce functional β-galactosidase
• Induce protein expression from inducible T7 RNA polymerase E. coli expression systems egulated by lac operon

Find the media format that fits your needs
We offer a wide variety of ready-to-use media formulations, such as LB Broth, Terrific Broth or S.O.C recovery medium.
X-Gal or Bluo-Gal for blue-white screening.
Explore other media formats for bacterial growth.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialIPTG (Isopropyl β-D-thiogalactopyranoside)
Quantity1 g
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
FormPowder
FormatBottle
Unit SizeEach
Contents & Storage
• IPTG Powder (1 g)

Store at –20°C.

Ready-to-use Bacterial Growth Media

Ready-to-use Bacterial Growth Media

See how these mediums can help with critical aspects of cloning!

Gibco LB Broth

Invitrogen S.O.C. Medium

Invitrogen One Shot LB Agar*

*Only available in North America and selected European countries

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