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Invitrogen™
UltraPure™ Salmon Sperm DNA Solution
UltraPure™ Salmon Sperm DNA Solution is a ready-to-use, sheared DNA solution that is used directly in the preparation of prehybridizationRead more
| Catalog Number | Quantity |
|---|---|
| 15632011 | 5 x 1 mL |
Catalog number 15632011
Price (EUR)
161,65
Online Exclusive
176,00Save 14,35 (8%)
Each
-
Quantity:
5 x 1 mL
Price (EUR)
161,65
Online Exclusive
176,00Save 14,35 (8%)
Each
UltraPure™ Salmon Sperm DNA Solution is a ready-to-use, sheared DNA solution that is used directly in the preparation of prehybridization and hybridization solutions. This DNA solution is prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free, RNase-free (DEPC-treated), distilled, deionized water. The DNA is sheared to an average size of ≤2,000 bp. The concentration is adjusted to 10 mg/mL.
Applications
Such solutions are used to block the nonspecific attachment of probe to the surface of a membrane.
Performance and quality testing
Concentration, size verification, viscosity, and ultraviolet absorbance (A260/A280) are analytically tested.
Applications
Such solutions are used to block the nonspecific attachment of probe to the surface of a membrane.
Performance and quality testing
Concentration, size verification, viscosity, and ultraviolet absorbance (A260/A280) are analytically tested.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Chromatin Biology, Nucleic Acid Gel Electrophoresis, Blotting
Key FunctionsBlock Non-Specific Hybridization
Product LineUltraPure™
Product TypeSalmon Sperm DNA Solution
Quantity5 x 1 mL
Reagent TypeSalmon Sperm DNA
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
5 tubes Salmon Sperm DNA solution (1 ml each, 10 mg/ml)
UltraPure™ Salmon Sperm DNA is stable for 1 year at -20°C.
UltraPure™ Salmon Sperm DNA is stable for 1 year at -20°C.
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Lot #Certificate TypeDateCatalog Number(s)
3183367Certificate of AnalysisJun 17, 202515632011
3136984Certificate of AnalysisMay 16, 202515632011
3168019Certificate of AnalysisMay 15, 202515632011
3124874Certificate of AnalysisMar 12, 202515632011
3122765Certificate of AnalysisFeb 18, 202515632011
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Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
The species of salmon is Oncerhyncus keta.
Ultrapure Salmon Sperm DNA is double stranded. It can be made single stranded by heating to 95 degrees C and then cooling rapidly on ice. Cooling is usually done with the DNA diluted in a hybridization solution which keeps it single stranded because of the large dilution volume.
Carrier DNA is typically used at a concentration of 100 µg/mL in both the prehybridization and hybridization solutions.
Citations & References (8)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
The human organic cation transporter (hOCT2) recognizes the degree of substrate ionization.
Journal:J Biol Chem
PubMed ID:11953440
'The organic cation transporter, OCT2, plays a role in renal secretion of a broad array of weak bases. To determine whether the degree of ionization of these compounds plays a role in their interaction with OCT2, we examined the influence of external pH values on the activity of the human
The role of polymer nanolayer architecture on the separation performance of anion-exchange membrane adsorbers: part II. DNA and virus separations.
Journal:Biotechnol Bioeng
PubMed ID:21618476
'The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the role of polymer nanolayer architecture on DNA and virus binding.
Spectrophotometric analysis of nucleic acids: oxygenation-dependent hyperchromism of DNA.
Journal:Anal Bioanal Chem
PubMed ID:20169336
'The absorbance at 260 nm (A(260)) is ubiquitously used for nucleic acid quantification. We show that following oxygenation, DNA solutions experience alterations in both spectral properties (hyperchromism in the UV region, lambda(max) 260 nm) and DNA conformation. The spectral changes caused by oxygen-DNA complexation are stable for at least several
A quantitative real-time polymerase chain reaction assay for the seagrass pathogen Labyrinthula zosterae.
Journal:Mol Ecol Resour
PubMed ID:21777400
'The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s.
Noncoding DNA in lipofection of HeLa cells-a few insights.
Journal:Mol Pharm
PubMed ID:23421924
'In cationic carrier-mediated gene delivery, the disproportional relationship between the quantity of delivered DNA and the amount of encoded protein produced is a well-known phenomenon. The numerous intracellular barriers which need to be overcome by pDNA to reach the nucleoplasm play a major role in it. In contrast to what
8 total citations