Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
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Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
Gibco™

Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has sinceRead more
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3176509210 x 500 mL
31765035500 mL
Catalog number 31765092
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447.65
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494.00
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Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Ham's F-12 is modified as follows:
With
• GlutaMAX™
• Phenol Red


The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco™ F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell LineCHO, COS-7, and rat prostate epithelial cells
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, GlutaMAX™
Product TypeHam's F-12 Nutrient Mixture
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutaMAX, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
3144468Certificate of AnalysisJun 06, 202531765035, 31765027
3137967Certificate of AnalysisApr 18, 202531765035, 31765027
3090881Certificate of AnalysisMar 22, 202531765035, 31765027
3082897Certificate of AnalysisFeb 19, 202531765035, 31765027
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Citations & References (3)

Citations & References
Abstract
The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain.
Authors: Kuroda Taruho S; Fukuda Mitsunori; Ariga Hiroyoshi; Mikoshiba Katsuhiko;
Journal:J Biol Chem
PubMed ID:11773082
rab27A, which encodes a small GTP-binding protein, was recently identified as a gene in which mutations caused human hemophagocytic syndrome (Griscelli syndrome) and ashen mice, which exhibit defects in melanosome transport as well as in regulated granule exocytosis in cytotoxic T lymphocytes. However, little is known about the molecular mechanism ... More
Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.
Authors: de Diego Iñaki; Schwartz Felix; Siegfried Heide; Dauterstedt Paul; Heeren Joerg; Beisiegel Ulrike; Enrich Carlos; Grewal Thomas;
Journal:J Biol Chem
PubMed ID:12070178
Annexins are Ca(2+)- and phospholipid-binding proteins that are widely expressed in mammalian tissues and that bind to different cellular membranes. In recent years its role in membrane traffic has emerged as one of its predominant functions, but the regulation of its intracellular distribution still remains unclear. We demonstrated that annexin ... More
Functional differences of tau isoforms containing 3 or 4 C-terminal repeat regions and the influence of oxidative stress.
Authors: Utton M A; Gibb G M; Burdett I D; Anderton B H; Vandecandelaere A;
Journal:J Biol Chem
PubMed ID:11438517
We report functional differences between tau isoforms with 3 or 4 C-terminal repeats and a difference in susceptibility to oxidative conditions, with respect to the regulation of microtubule dynamics in vitro and tau-microtubule binding in cultured cells. In the presence of dithiothreitol in vitro, a 3-repeat tau isoform promotes microtubule ... More
3 total citations

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