Pierce™ Fast Western Blot Kit, ECL Substrate
Pierce™ Fast Western Blot Kit, ECL Substrate
Thermo Scientific™

Pierce™ Fast Western Blot Kit, ECL Substrate

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to performRead more
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Catalog NumberQuantity
35050Promo Image1 L Kit
35055Promo Image200 mL Kit
Catalog number 35050
Price (USD)
307.65
Online Exclusive
359.00
Save 51.35 (14%)
Each
In stock
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Quantity:
1 L Kit
Recurring order eligible. Learn more »
Price (USD)
307.65
Online Exclusive
359.00
Save 51.35 (14%)
Each
Add to cart
Ask our AI about this Product
The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ConjugateHRP
ReactivityMouse, Rabbit
Sufficient For25 Mini Blots
Detection MethodChemiluminescence
For Use With (Application)Western Blot
FormLiquid
Membrane CompatibilityNitrocellulose, PVDF
Product LinePierce™
Product TypeWestern Blot Kit
Quantity1 L Kit
SubstrateHorseradish Peroxidase
Unit SizeEach
Contents & Storage
Sufficient For: 25 mini blots probed with mouse or rabbit primary antibodies
• Fast Western Antibody Diluent, 500 mL
• Fast Western 10X Wash Buffer, 250 mL
• Fast Western Optimized HRP Reagent, 25 mL
• Pierce ECL Detection Reagent 1, 125 mL
• Pierce ECL Detection Reagent 2, 125 mL

Store at 4°C.
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Frequently asked questions (FAQs)

Does my primary antibody need to be conjugated for using with the Pierce Fast Western Blot Kit, ECL Substrate (Cat. No. 35050, 35055)?

No, your primary antibody does not need to be conjugated for using with the Pierce Fast Western Blot Kit, ECL Substrate (cat. No. 35050, 35055). The kit includes all necessary reagents except the primary antibody, and it works with both mouse and rabbit primary antibodies. The HRP reagent directly binds to both mouse and rabbit primary antibodies, thus, a secondary antibody is not needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

For western blotting with the Pierce Fast Western Blot Kit, ECL Substrate (Cat. No. 35050), do I need a secondary antibody?

The kit contains the horseradish peroxidase (HRP) reagent and ECL detection reagent, therefore the kit works with blots probed with mouse or rabbit primary antibodies. The HRP reagent directly binds to both mouse or rabbit primary antibodies, thus, a secondary antibody is not needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3214912Certificate of AnalysisJul 03, 202535055
3215041Certificate of AnalysisJun 02, 202535050
3233313Certificate of AnalysisJun 02, 202535050
3212250Certificate of AnalysisMay 07, 202535050
AC408079Certificate of AnalysisApr 04, 202535055
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