Pierce™ ECL Western Blotting Substrate

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Pierce™ ECL Western Blotting Substrate

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) thatRead more

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Catalog Number	Quantity
32106	500 mL
32109	50 mL
32209X4	4 x 250 mL
32209	250 mL
32106X4	4 x 500 mL

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Catalog number 32106

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271.65

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310.00

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500 mL

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Price (USD)

271.65

Online Exclusive

310.00

Save 38.35 (12%)

Each

Add to cart

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›

Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ConjugateHRP

DescriptionPierce ECL Western Blotting Substrate

Kit ContentsLuminol/Enhancer, 250mL, Stable Peroxide Buffer, 250mL

Recommended Antibody Concentrations2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)

SensitivityLow Picogram

Signal Duration1 to 2 hr.

Sufficient For5000 sq. cm

Detection MethodChemiluminescence

For Use With (Application)Western Blot

FormLiquid

Membrane CompatibilityNitrocellulose, PVDF

Product LinePierce™

Product TypeSubstrate

Quantity500 mL

SubstrateHorseradish Peroxidase

Unit SizeEach

Contents & Storage

Upon receipt store at 2-8°C. Product is shipped at ambient temperature.

Introducing iBlot 3 Western Blot Transfer System

Featuring higher throughput and built-in cooling for consistent protein transfer
Learn more ›

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Frequently asked questions (FAQs)

How much more sensitive is SuperSignal West Femto Maximum Sensitivity Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Femto Maximum Sensitivity Substrate is about 25- to 50-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Femto Maximum Sensitivity Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip # 32 Guide to Enzyme Substrates for Western Blotting (https://tools.thermofisher.com/content/sfs/brochures/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much more sensitive is SuperSignal West Dura Extended Duration Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.