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TaqMan™ MGB Probe
TaqMan™ MGB Probe
Applied Biosystems™

TaqMan™ MGB Probe

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Custom TaqMan™ probes are dual-labeled primers suitable for use in any real-time PCR applicaton. They incorporate a 5' reporter dyeRead more
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Catalog NumberQuantity
4316034Promo Image6,000 pmol Scale
4316032Promo Image50,000 pmol Scale
4316033Promo Image20,000 pmol Scale
Catalog number 4316034
Price (USD)
394.65
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419.00
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Custom TaqMan™ probes are dual-labeled primers suitable for use in any real-time PCR applicaton. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/Minor Groove Binder (MGB). Choose among FAM™, VIC™, TET™, and NED™ as reporters. Probes are HPLC-purified.

Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5' fluorescent reporter dye and a 3' nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. Custom TaqMan MGB probes are HPLC-purified and available with FAM, VIC, TET, and NED reporter dyes.

Shorter, more specific probes

TaqMan MGB probes include a MGB moiety at the 3' end that increases the melting temperature (Tm) of the probe and stabilizes probe/target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.

Nonfluorescent quencher (NFQ) maximizes sensitivity

TaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.

Guaranteed yield

The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.

Approximate ship time

4–6 days in North America, 4-7 days in Europe

We also offer:

  • Applied Biosystems custom primers and other types of TaqMan probes.
  • TaqMan MGB probes and primers available as predesigned and pre-formulated TaqMan Assays.
  • Commercial manufacturing products and services, including OEM and GMP oligo manufacturing.

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
3'Primer ModificationMGB-NFQ
5'Primer ModificationFAM, NED, TET, VIC
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
FormatTube
Green FeaturesSustainable packaging
Internal Probe ModificationNone
Product LineTaqMan™
Product TypeTaqMan Probe
Purification MethodHPLC
Purity or Quality GradeHPLC
Quantity6,000 pmol Scale
Shipping ConditionRoom Temperature
For Use With (Application)Real Time PCR (qPCR)
FormLiquid
Label or DyeFAM
Unit SizeEach
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Frequently asked questions (FAQs)

I would like to calibrate my Real-Time PCR instrument with a custom dye, which buffer should I use?

Please ask your dye manufacturer for recommendations. If there is no specific recommendation available, try TE Buffer (pH 8.0) to start.

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I determine whether the Tm of my custom probe is appropriate?

In general, the target Tm of a TaqMan probe is 70 degrees C. Using the Primer Express Software, you can check the Tm of a MGB or TAMRA probe probe.

Within the software, go to Primer Probe Test Tool and then copy/paste in your sequence of interest to see the Tm. If you need to check for a MGB based probe, make sure to choose Document Type: TaqMan MGB Quantification. If you need to check for a QSY or TAMRA based probe, make sure to choose Document Type: TaqMan Quantification. Real-Time PCR and Digital PCR Applications

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

I have purchased custom oligos (primers and probes) and I want to make a 20x working stock for a TaqMan assay, similar to the pre-designed assays. How do I dilute and mix my oligos to make a 20x working stock?

Most pre-designed TaqMan assays are supplied as a 20x assay concentrate containing 18 uM of each primer and 5 uM of probe which provides a 900 nM of each primer and 250 nM of the probe (for gene expression assays) and 200 nM of each probe (for SNP genotyping assays) at the final 1x concentration.

In order to make a 20x working stock from custom oligos, the oligos need to be diluted as follows: To make a 20x Assay stock for Gene Expression assays, make 100 uM stocks of both forward and reverse primers, and TaqMan probe. Add 18 ul of each primer and 5 ul of the TaqMan probe, which gives 41 ul volume. Add 59 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan Gene Expression Assay. To make a 20x Assay stock for a Genotyping Assay, make 100 uM stocks of both forward and reverse primers, and both TaqMan probes. Add 18 ul of each primer and 4 ul of each TaqMan probe, which gives 44 ul volume. Add 56 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan SNP Genotyping Assays.

I am looking to order a molecular probe for validation, using specific sequences. How do I order a custom probe?

We do not offer any custom probes for diagnostic purposes.

We do offer custom TaqMan probes for qPCR experiments, which are for Research Use Only.
TaqMan MGB Probe
TaqMan QSY Probe
TaqMan TAMRA Probe
These above probes can be designed in our online tool, Custom TaqMan Probes.

If you would like to order a pre-mix of primers and probe, please check our custom TaqMan Assays.
You can find more information one the Custom TaqMan Gene Expression Assay page on thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
7359572Certificate of AnalysisOct 05, 20204316033
7346277Certificate of AnalysisSep 01, 20204316033
7301934Certificate of AnalysisJul 02, 20204316033
7257960Certificate of AnalysisMay 05, 20204316033
7209853Certificate of AnalysisMar 09, 20204316033
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