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Pierce™ Immobilized Reductant Column, 2 mL
Pierce™ Immobilized Reductant Column, 2 mL
Thermo Scientific™

Pierce™ Immobilized Reductant Column, 2 mL

The Thermo Scientific Pierce Immobilized Reductant Column contains beaded resin to which an thiol-based reducing agent has been immobilized toRead more
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Catalog NumberQuantity
777011 column
Catalog number 77701
Price (USD)
474.00
Each
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Quantity:
1 column
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Price (USD)
474.00
Each
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The Thermo Scientific Pierce Immobilized Reductant Column contains beaded resin to which an thiol-based reducing agent has been immobilized to enable solid-phase reduction of peptide and protein disulfide bonds.

Features of the Immobilized Reductant Column:

• Eliminates soluble components which interfere with the assay of free thiol groups
• No need to remove reducing agent after the reaction
• Reduced protein, free of contaminating thiols, can be easily assayed using the BCA method
• Solid phase; protocols for both peptides and proteins
• Excellent protein recovery
• Regenerable matrix useable up to four times

Both the mercaptan and the DTT methods of reduction use soluble reducing agents. This requires that the reducing reagents be removed from the sample before further procedures can be performed. Measurements of free sulfhydryl content cannot be made in the presence of these agents, and they must be removed prior to crosslinking. Removal of a soluble reductant can be difficult when a peptide is involved, and extensive desalting may result in protein loss or extreme dilution when small quantities of protein are reduced.

Pierce Immobilized Reductant Columns were developed to eliminate these difficulties, and are highly effective for reducing disulfide bonds. Immobilized TCEP Disulfide Reducing Gel is also available for reduction of peptides and proteins without contaminating your sample with reducing agent.

Related Products
Pierce™ Immobilized TCEP Disulfide Reducing Gel
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid
Product LinePierce™
Quantity1 column
Product TypeReductant Column
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
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Lot #Certificate TypeDateCatalog Number(s)
ZG397181Certificate of AnalysisAug 07, 202477701
YK384965Certificate of AnalysisDec 08, 202377701
XB331984Certificate of AnalysisMay 17, 202277701
WC318516Certificate of AnalysisApr 07, 202177701
VJ313044Certificate of AnalysisOct 12, 202077701
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