GeneChip™ Eukaryotic Poly-A RNA Control Kit

The GeneChip™ Eukaryotic Poly-A RNA Control Kit is used with the Prokaryotic Target Labeling Assay and the Prokaryotic Target LabelingRead more

Catalog Number	Quantity
900433	1 kit

Catalog number 900433

Price (USD)

Quantity:

1 kit

The GeneChip™ Eukaryotic Poly-A RNA Control Kit is used with the Prokaryotic Target Labeling Assay and the Prokaryotic Target Labeling Assay for 3' expression studies with prokaryotic GeneChip Brand Arrays. The assays utilize reverse transcriptase and random hexamer primers to produce DNA complementary to the RNA. The cDNA products are then fragmented by DNase I and labeled with terminal transferase and biotinylated GeneChip DNA Labeling Reagent at the 3' termini.

Functionally tested on GeneChip Brand Arrays, we recommend the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.

Prokaryotic Target Labeling Assay components include:
• B2 Control Oligo (3nM)
• GeneChip DNA Labeling Reagent
• GeneChip Poly-A RNA Control Kit

Related Links
GeneChip™ Wash Buffer A
GeneChip™ Wash Buffer B

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeOligonucleotides, Primers

Quantity1 kit

PrimerRandom Hexamer

Product LineGeneChip™

Unit SizeEach

The Poly-A Control Dil Buffer comes in one big bottle of 3.8 mL and it is inconvenient to freeze/thaw each time since it freezes when stored at -20 degrees C. What do you recommend and can you change the packaging so the buffer comes in smaller aliquots?

Where can I find the protocol for diluting the Poly-A Control Stock before adding to the samples?

Should I anticipate the same results using the Poly-A controls with all eukaryotic GeneChip expression arrays?

At what temperature should the kit be stored?

Can this kit be used with prokaryotic samples and arrays?

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Frequently asked questions (FAQs)

What are the estimated Poly-A RNA Control final concentrations when processing expression arrays?

Here are the Poly-A RNA Controls final concentrations (in samples):
- lys 1:100,000
- phe 1:50,000
- thr 1:25,000
- dap 1:7,500

What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?

The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).

Can I purchase one, or all, of the four Poly-A Controls separately?

These four controls are only available as a pre-mixed cocktail with the defined concentrations and relative ratios. This combination of concentrations has been tested to provide qualitative information on both sensitivity and dynamic range of the labeling assay. Providing these controls in a pre-mixed format increases ease-of-use and reduces the number of manual dilution steps.
Information on ordering individual poly-A control clones and preparing poly-A stocks is available in Section 2, Chapter 2, and Section 3, Chapter 2, of the GeneChip Expression Analysis Technical Manual.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I purchase the Poly-A Control Stock and the Poly-A Control Dil Buffer separately?

The Control Stock and the Dilution Buffer are packaged in the same Kit to provide convenience to customers. Currently they cannot be purchased as separate products.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Do you have any specific recommendation on the type of non-stick tubes to use for diluting the Poly-A RNA Control Stock?

The following tubes were used in-house during product development of this Poly-A RNA Control Kit, and we have not tested any other tubes at this time:
Non-stick RNase-free 0.5 mL microfuge tubes, Ambion, Cat. No. 12350
Non-stick RNase-free 1.5 mL microfuge tubes, Ambion, Cat. No. 12450

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

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