CTS™ OpTmizer™ T Cell Expansion SFM, bottle format
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CTS™ OpTmizer™ T Cell Expansion SFM, bottle format
Gibco™

CTS™ OpTmizer™ T Cell Expansion SFM, bottle format

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™Read more
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Catalog NumberQuantity
A10485011000 mL
Catalog number A1048501
Price (USD)
205.00
Each
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Quantity:
1000 mL
Price (USD)
205.00
Each
Add to cart
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GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium
• Consistent performance in supporting T cell expansion
• Supports high density T cell culture (e.g., >3x106 CD3+ T cells/mL) in static culture

With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.

Workflow benefits:
• Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs
• More rapid T cell expansion, thus reducing cycle time and improving productivity
• Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload
Specifications
Cell TypeT Cells
Culture EnvironmentCO2
Endotoxin LevelVery Low
Included AntibioticsNo antibiotics
Inorganic SaltsCalcium, Magnesium
Product LineCTS™, OpTmizer™
Product TypeCell Expansion Serum Free Medium (SFM)
Purity or Quality GradeResearch Grade
Quantity1000 mL
ClassificationSerum-free, Xeno-free
Culture TypeSuspension Cell Culture
FormLiquid
Serum LevelSerum-free
SterilitySterile
With AdditivesHEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Without AdditivesNo Glutamine
Unit SizeEach
Contents & Storage
OpTmizer™ T-Cell Expansion Basal Medium (1 x 1000 mL bottle): Store at 2–8°C. Protect from light.

OpTmizer™ T-Cell Expansion Supplement (1 x 26 mL): Store in the dark at 2–8°C.
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Frequently asked questions (FAQs)

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to order additional components with the CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503)?

The CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) kit comes with OpTmizer T Cell Expansion Basal Medium and OpTmizer T Cell Expansion Supplement, which are mixed together prior to use. Depending on your protocol you may need to purchase T cell growth factors such as IL-2 (Cat. No. PHC0023) and L-glutamine (Cat. No. 25030) to form a complete medium. To boost growth of human T cells, human AB serum can be added. If desired, antibiotics such as Gentamicin (Cat. No. 15750), can also be added.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) work with all types of T cells?

CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has been demonstrated to be effective for the ex vivo expansion of human CD3 T cells, including polyclonal T cell populations, antigen-specific T cells, tumor-infiltrating lymphocytes (TIL), cord blood T cells, regulatory T cells, T cells from HIV-infected donors, gene-modified T cells (both lenti- and MLV-based vectors), including cells of both the CD4+ and CD8+ phenotype.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) be used for animal T cell cultures other than human?

CTS OpTmizer T Cell Expansion SFM (Cat. No. A1048501, A1048502, A1048503) has only been tested on human T cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a difference in formulation of the CTS OpTmizer T Cell Expansion SFM between the bottle and bag packaging formats?

The bottle and bag packaging formats of the medium have the same formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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