StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Cell lines tested for Accutase® application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells. A complete list of published references is available on our website. Accutase® is a trademark of ICT, Inc.
Specifications
Cell Type
Stem Cells
Classification
Serum-free
Culture Type
Adherent Cell Culture
Form
Frozen
Product Type
Cell Culture Dissociation Reagent
Serum Level
Serum-Free
Shelf Life
24 Months
Inorganic Salts
Sodium Phosphate
pH
6 to 8
Product Line
Gibco™, StemPro™
Purity or Quality Grade
Research Grade
Quantity
100 mL
Shipping Condition
Dry Ice
Source
Serum-Free
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.