GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells
GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells
Invitrogen™

GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNARead more
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Catalog NumberQuantity
A4663310 Reactions
Catalog number A46633
Price (USD)
239.00
Each
In stock
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Quantity:
10 Reactions
Price (USD)
239.00
Each
Add to cart
The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells, is a complete kit that includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.

Features of the GeneArt Gibson Assembly EX Cloning Kit include:
Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
Efficient—robust efficiency provides successful clones for both simple and challenging constructs
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments
GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly EX kits are available in multiple formats, including:
• GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells) (this page)
GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
GeneArt Gibson Assembly EX Master Mix kits (cloning kit without the cells)

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bacterial or Yeast StrainTOP10
Cell TypeChemically Competent E. coli
Cloning MethodSeamless cloning
Control TypePositive Control
For Use With (Application)Seamless Cloning & Genetic Assembly
Number of FragmentsUp to 15 fragments
Product LineGENEART™
Product TypeCloning Kit
Quantity10 Reactions
Shipping ConditionDry Ice
Workflow StepDNA Assembly
FormatKit
Unit SizeEach
Contents & Storage
Includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.

Ready-to-use Bacterial Growth Media

Ready-to-use Bacterial Growth Media

See how these mediums can help with critical aspects of cloning!

Gibco LB Broth

Invitrogen S.O.C. Medium

Invitrogen One Shot LB Agar*

*Only available in North America and selected European countries

Frequently asked questions (FAQs)

How big do DNA fragment homology arms need to be when using Gibson Assembly kits?

For fragments that are 8 kb or lesser in length, an overlap of 20-40 bp is recommended. For fragments greater than 8 kb in length, an overlap of 40-100 bp is recommended.

What type of vector can I use with Gibson Assembly kits?

Any linearized vector can be used with Gibson Assembly kits. We recommend using high-copy vectors for small inserts (<10 kb) and low-copy vectors for large inserts or toxic genes.

What type of inserts can I use with Gibson Assembly kits?

Any linearized DNA fragment, including PCR products, restriction digestion products, and GeneArt Strings can be used with Gibson Assembly kits.

What is the difference between the Gibson Assembly HiFi and the Gibson Assembly EX kits?

Gibson Assembly HiFi kits provide the quickest and easiest way to assemble up to six fragments in a single-tube reaction. The more robust Gibson Assembly EX kits enable assembly of up to 15 fragments in a single-tube, two-step reaction.

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