Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Product Type | Stand-alone enzyme |
For Use With (Application) | LAMP, RT-LAMP |
Polymerase | Lyo-ready Bst DNA Polymerase |
Quantity | 6000 U |
No. of Reactions | 1000 Reactions |
Optimal Reaction Temperature | 65°C |
Shipping Condition | Dry Ice |
Concentration | 6 U/μL |
Reaction Time | ≤10 minutes |
Unit Size | Each |
Product Type | Stand-alone enzyme |
For Use With (Application) | LAMP, RT-LAMP |
Polymerase | Lyo-ready Bst DNA Polymerase |
Quantity | 1200 U |
No. of Reactions | 200 Reactions |
Optimal Reaction Temperature | 65°C |
Shipping Condition | Dry Ice |
Concentration | 6 U/μL |
Reaction Time | ≤10 minutes |
Unit Size | Each |
Product Type | Stand-alone enzyme |
For Use With (Application) | LAMP, RT-LAMP |
Polymerase | Lyo-ready Bst DNA Polymerase |
Quantity | 1200 U |
No. of Reactions | 200 Reactions |
Optimal Reaction Temperature | 65°C |
Shipping Condition | Dry Ice |
Concentration | 40 U/μL |
Reaction Time | ≤10 minutes |
Unit Size | Each |
Catalog Number | Specifications | Unit Size | Concentration | Quantity | Price (USD) | Availability | Quantity | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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A56656 | Each | 6 U/μL | 6000 U | 275.00 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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A56655 | Each | 6 U/μL | 1200 U | 66.50 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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A56657 | Each | 40 U/μL | 1200 U | 66.50 | - | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).
Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.
Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs
Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)
Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
• Lyo-ready Bst DNA Polymerase (5 x 200 μL at 6 U/μL)
• 10X Bst Reaction Buffer (3 x 1 mL)
• 200 mM MgCl2 (1 mL)
• 5 M Betaine (3 x 1 mL)
Store at –15 to –25°C.