The 10X FastDigest Green Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Green Buffer. Therefore, enzymes used in downstream applications can be directly added to the FastDigest reaction mix

Benefits:
• Double and multiple digestion in a single buffer with any combination of enzymes.
• No sequential digestions or buffer changes needed.
• Allows direct loading of reaction mixture on gels.
• Saves pipetting time and eliminates the need for DNA loading dyes.