HPF (Hydroxyl Radical and Peroxynitrite Sensor)
HPF (Hydroxyl Radical and Peroxynitrite Sensor)
Invitrogen™

HPF (Hydroxyl Radical and Peroxynitrite Sensor)

Aminophenyl fluorescein (APF, A36003) and hydroxyphenyl fluorescein (HPF), two reactive oxygen species (ROS) indicators developed by T. Nagano, offer greaterRead more
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Catalog NumberQuantity
H36004470 μL
Catalog number H36004
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694.65
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716.00
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Quantity:
470 μL
Price (USD)
694.65
Online Exclusive
716.00
Save 21.35 (3%)
Each
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Aminophenyl fluorescein (APF, A36003) and hydroxyphenyl fluorescein (HPF), two reactive oxygen species (ROS) indicators developed by T. Nagano, offer greater specificity and stability than dichlorodihydrofluorescein diacetate (H2DCFDA, D399). APF and HPF show limited non-selective reactivity and relatively high resistance to light-induced oxidation. Both of these new fluorescein derivatives are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion; APF will also react with the hypochlorite anion. Together, these two ROS indicators can selectively detect hypochlorite anion. Upon oxidation, both APF and HPF exhibit bright green fluorescence (excitation/ emission maxima ∼490/515 nm), making them compatible with any instrument that can detect fluorescein.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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Figures

Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3143946Certificate of AnalysisMar 30, 2025H36004
2896550Certificate of AnalysisApr 24, 2024H36004
2831244Certificate of AnalysisJan 31, 2024H36004
2533877Certificate of AnalysisDec 05, 2022H36004
2395120Certificate of AnalysisOct 09, 2021H36004
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Citations & References (30)

Citations & References
Abstract
Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
Authors:Halliwell B, Whiteman M
Journal:Br J Pharmacol
PubMed ID:15155533
'Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative ... More
Externally applied pressure activates pancreatic stellate cells through the generation of intracellular reactive oxygen species.
Authors:Asaumi H, Watanabe S, Taguchi M, Tashiro M, Otsuki M,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:17761838
'Local tissue pressure is higher in chronic pancreatitis than in the normal pancreas. We reported recently that pressure application induces synthesis of extracellular matrix (ECM) and cytokines in pancreatic stellate cells (PSCs) and that epigallocatechin gallate (EGCG), a potent antioxidant, inhibits the transformation of PSCs from quiescent to activated phenotype ... More
The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia.
Authors:Tomizawa S, Imai H, Tsukada S, Simizu T, Honda F, Nakamura M, Nagano T, Urano Y, Matsuoka Y, Fukasaku N, Saito N
Journal:Neurosci Res
PubMed ID:16168507
'A novel fluorescence probe, 2-[6-(4''-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (HPF) was used to investigate the generation of highly reactive oxygen species (hROS) under ischemia both in vitro and in vivo. In the in vitro study, HT 22 cells were used to demonstrate that was predominantly detected in the cytoplasm, which coincides ... More
Time to fatigue is increased in mouse muscle at 37 degrees C; the role of iron and reactive oxygen species.
Authors:Reardon TF, Allen DG,
Journal:J Physiol
PubMed ID:19470779
'Studies exploring the rate of fatigue in isolated muscle at 37 degrees C have produced mixed results. In the present study, muscle fibre bundles from the mouse foot were used to study the effect of temperature on the rate of muscle fatigue. Provided iron was excluded from the solutions, time ... More
Fluorescence probes used for detection of reactive oxygen species.
Authors:Gomes A, Fernandes E, Lima JL,
Journal:J Biochem Biophys Methods
PubMed ID:16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
30 total citations

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