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Thermo Scientific™
GeneJET Genomic DNA Purification Kit
Catalog number: K0722
Have Questions? 
Thermo Scientific™
GeneJET Genomic DNA Purification Kit
Catalog number: K0722
Have Questions? Catalog Number
K0722
Unit Size
250 preps
Price (USD)
566.65 Online price
714.00
Availability
-
Quantity
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| K0722 | 250 preps | 566.65 Online price 714.00 | - | ||
| K0721 | 50 preps | 128.65 Online price 162.00 | - |
Product Overview
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Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes
Applications
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Sample Type
Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Scale
Mini
Elution Volume
200 μL
Final Product Type
gDNA
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Test Time
20 min. (After sample homogenization)
Yield
≤30 μg
Starting Material Amount
Bacteria: ≤109 cells
Cells: ≤106
Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)
Tissue: ≤20 mg
Whole Blood: ≤200 μL
Yeast: ≤108 cells
Cells: ≤106
Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)
Tissue: ≤20 mg
Whole Blood: ≤200 μL
Yeast: ≤108 cells
Contents & Storage
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening
• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening
• 55 mL Digestion Solution; room temperature
• 2 x 60 mL Lysis Solution; room temperature
• 40 mL Wash Buffer I (concentrated); room temperature
• 40 mL Wash Buffer II (concentrated); room temperature
• 150 mL Elution Buffer; room temperature
• 250 GeneJET Purification Columns with Collection Tubes; room temperature
• 250 Collection Tubes; room temperature
• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening
• 55 mL Digestion Solution; room temperature
• 2 x 60 mL Lysis Solution; room temperature
• 40 mL Wash Buffer I (concentrated); room temperature
• 40 mL Wash Buffer II (concentrated); room temperature
• 150 mL Elution Buffer; room temperature
• 250 GeneJET Purification Columns with Collection Tubes; room temperature
• 250 Collection Tubes; room temperature
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